PMID: 9662487Jul 15, 1998Paper

Alterations in renal degradation of albumin in early experimental diabetes in the rat: a new factor in the mechanism of albuminuria

Clinical Science
M J BurneW D Comper

Abstract

1. Albumin is normally excreted as a mixture of intact protein and fragments that are produced during renal passage. The purpose of this study was to investigate the ratio of intact versus degraded forms of excreted albumin to ascertain whether changes in this ratio could account for the apparent increase in albumin excretion seen in diabetes, as measured by standard radioimmunoassay techniques. 2. Four-week male Sprague-Dawley rats with streptozotocin-induced diabetes and age-matched control rats were intravenously injected with [3H]albumin. Urine collected over 2 h was analysed by size exclusion chromatography and radioimmunoassay. A standard radioimmunoassay found a 7-fold increase in albumin excretion rate in diabetic rats, whereas there was only a 2-fold increase in albumin excretion (intact plus fragments). Urine analysed by size exclusion chromatography showed severe degradation for control rats (% monomer=4+/-2%); in diabetic rats there was a significant amount of monomer albumin excreted, along with moderately degraded and heavily degraded albumin (% monomer=17+/-5%). 3. This study has shown that the radioimmunoassay, which specifically detects intact albumin, considerably underestimates the amount of total urinary alb...Continue Reading

Citations

May 20, 2005·The Journal of Laboratory and Clinical Medicine·Kimberley J StrongWayne D Comper
May 20, 2004·Kidney International·Krishnamurthy P GudehithluAshok K Singh
Jan 28, 2012·Journal of the American Society of Nephrology : JASN·Kathrin WeyerHenrik Birn
Oct 1, 2008·Indian Journal of Nephrology·M PrakashR Prabhu
Jul 24, 2010·Clinical and Experimental Nephrology·Kiyoshi MoriKazuwa Nakao
Sep 4, 2010·Journal of Stroke and Cerebrovascular Diseases : the Official Journal of National Stroke Association·Peter TothIstvan Wittmann
Jan 20, 2009·Clinica Chimica Acta; International Journal of Clinical Chemistry·Aki NakayamaKiyoko Shiba
Jun 30, 2001·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·K A GreiveW D Comper
Nov 13, 2004·The Journal of Clinical Hypertension·Douglas E Busby, George L Bakris
Jan 21, 2006·Clinica Chimica Acta; International Journal of Clinical Chemistry·John H ContoisMichael J Thompson
Jan 31, 2006·The Journal of Laboratory and Clinical Medicine·Lucinda M HilliardWayne D Comper
Apr 12, 2005·Advances in Chronic Kidney Disease·Wayne D Comper, Tanya M Osicka

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.