Alterations in serum microRNA in humans with alcohol use disorders impact cell proliferation and cell death pathways and predict structural and functional changes in brain

BMC Neuroscience
Cherry IgnacioFrank A Middleton

Abstract

There is currently a lack of reliable, minimally invasive biomarkers that could predict the extent of alcoholism-induced CNS damage. Developing such biomarkers may prove useful in reducing the prevalence of alcohol use disorders (AUDs). Extracellular microRNAs (miRNAs) can be informative molecular indicators of changes in neuronal gene expression. In this study, we performed a global analysis of extracellular miRNAs to identify robust biomarkers of early CNS damage in humans diagnosed with DSM-IV AUDs. We recruited a relatively young set of 20 AUD subjects and 10 age-matched controls. They were subjected to comprehensive medical, neuropsychological and neuroimaging tests, followed by comparison of miRNA levels found in peripheral blood serum. Employing a conservative strategy to identify candidate biomarkers, miRNAs were quantified using two independent high-throughput methods: microarray and next-generation RNA-sequencing. This improved our capacity to discover and validate relevant miRNAs. Our results identified several miRNAs with significant and reproducible expression changes in AUD subjects versus controls. Moreover, several significant associations between candidate miRNA biomarkers and various medical, neuropsychologica...Continue Reading

Associated Datasets

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Citations

Nov 28, 2015·Biomolecules·Sathish Kumar NatarajanJustin L Mott
Apr 4, 2016·Alcoholism, Clinical and Experimental Research·Amy S GardinerNora I Perrone-Bizzozero
Aug 19, 2016·International Journal of Molecular Sciences·Rocío BarragánDolores Corella
Mar 28, 2017·Progress in Neurobiology·Julianna KardosMiklós Palkovits
Aug 26, 2017·Pharmacogenomics·Cynthia Marie-ClaireFlorence Vorspan
Nov 10, 2018·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Stefania CiafrèMauro Ceccanti
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Feb 6, 2017·Cell Stress & Chaperones·Zhongkai WangXianxian Zhao
Mar 20, 2019·Journal of Experimental Neuroscience·Hamna Atif, Steven D Hicks
Oct 18, 2019·Journal of Controlled Release : Official Journal of the Controlled Release Society·Sharon Wei Ling LeeValeria Chiono

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Datasets Mentioned

BETA
GSE71579
and

Methods Mentioned

BETA
RNA-Seq
Chip

Software Mentioned

Strand NGS suites
Core Analysis package
MEV4
Fiji
QIAGEN Ingenuity IPA
FreeSurfer
Microarray Experiment Viewer 4 ( MEV4 )
Partek Genomics Suite
MEV
Partek Flow

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