Alterations in serum thrombospondin in patients with amyotrophic lateral sclerosis

Journal of the Neurological Sciences
I V Smirnova, B W Festoff

Abstract

Thrombospondin (TSP), an endogenous extracellular matrix (ECM) glycoprotein, is secreted from platelet alpha-granules after thrombin stimulation. Alterations in blood TSP levels occur in different pathologic conditions, suggesting it is a marker for certain disorders. We previously found a marked increase in TSP deposition in the muscle ECM of patients with amyotrophic lateral sclerosis (ALS) in comparison with controls. Because the mechanism for this increase is unknown, we compared serum TSP levels in 11 patients to 15 controls using three different site-specific monoclonal antibodies (MA-I, MA-II and A6.1). We found mean serum TSP concentrations by indirect ELISA to be significantly decreased in the ALS patients. Using laser densitometry we calculated the ratio of fragmented to native TSP from Western immunoblots probed with A6.1, where a higher ratio corresponds to increased fragments. Mean values for this ratio were 6.3 +/- 4.9 and 18.3 +/- 8.2 for controls and patients, respectively. Thus significant decrease in native TSP and increase in its proteolytic fragments in ALS is consistent with increased proteolytic enzyme activity. Dysregulation of the protease: inhibitor balance in this degenerative condition may be reflecte...Continue Reading

References

Nov 1, 1992·Cancer Metastasis Reviews·D A Walz
Nov 1, 1992·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·P Bornstein
Sep 1, 1992·Thrombosis Research·C Leroy-MatheronM Gouault-Heilmann
Feb 1, 1991·Thrombosis Research·A WehmeierW Schneider
Feb 4, 1992·Biochemistry·J LawlerM Duquette
Mar 1, 1991·The Journal of Cell Biology·C A PraterW A Frazier
Feb 1, 1989·Journal of the Neurological Sciences·N Adachi, S Shoji
Feb 1, 1989·European Journal of Cancer & Clinical Oncology·D A PrattJ Dawes
Jan 1, 1989·Methods in Enzymology·H S Slayter
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A S AschR L Nachman
Jan 1, 1986·Annals of the New York Academy of Sciences·J LawlerL H Derick
Apr 1, 1985·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·T N WightP Bornstein
May 1, 1982·The Journal of Cell Biology·D F MosherE A Jaffe
Jan 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology

❮ Previous
Next ❯

Citations

Apr 20, 2004·The International Journal of Biochemistry & Cell Biology·Josephine C Adams
Jun 18, 2002·Journal of Neurochemistry·Ralph W KunclS Patricia Becerra
Jan 11, 2013·The International Journal of Neuroscience·Madhuri Behari, Mohita Shrivastava
Dec 10, 1999·Molecular and Cellular Neurosciences·J FritscheC E Bandtlow
Feb 15, 2001·The Journal of Comparative Neurology·I A ScarisbrickM Rodriguez
May 10, 2008·Expert Review of Proteomics·Henrik Ryberg, Robert Bowser
Sep 7, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·G TarabolettiM Ziche
May 21, 2020·Frontiers in Immunology·Odette Leiter, Tara L Walker
Feb 26, 2019·Frontiers in Neurology·Barry W Festoff, Bruce A Citron
Jun 25, 2003·The Journal of Biological Chemistry·Zhiming SuoBarry W Festoff

❮ Previous
Next ❯

Related Concepts

Related Feeds

Amyloid Lateral Sclerosis

Amyotrophic Lateral Sclerosis (ALS) is a progressive nervous system disease associated with the death of neurons that control voluntary muscles. Discover the latest research on ALS here.