Alterations in spinal cord gene expression after hindpaw formalin injection

Journal of Neuroscience Research
Xiangqi LiJ David Clark

Abstract

Heme oxygenase type 2 (HO-2) is an enzyme that uses heme as a substrate to produce iron, biliverdin, and carbon monoxide (CO). This enzyme participates in regulation of nociceptive signal transmission in spinal cord tissue. We set out to identify genes undergoing alterations in expression in a model of inflammatory pain and to determine whether HO-2 participates in that regulation. After the hindpaw injection of formalin in mice, we measured changes in expression of immediate early genes including c-fos, c-jun, jun B, nerve growth factor induced genes (NGFI-A and NGFI-B) and activity-related cytoskeletal protein (ARC) using real-time PCR. The mRNA corresponding to these genes increased in abundance in the first hour after formalin injection and then slowly declined. Changes in the abundance of prodynorphin, extracellular signal related kinases (ERK1 and ERK2) and N-methyl-D-aspartate (NMDA) receptor R1 subunit mRNA generally peaked between 8 and 12 hr after formalin injection. In HO-2 null mutant mice, the enhancement of expression was less for all genes studied. We went on to quantify gene expression in superficial dorsal horn tissue using laser capture microdissection followed by RNA amplification and real-time PCR. The resul...Continue Reading

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Citations

Feb 14, 2009·Pharmacogenetics and Genomics·Larry F ChuJ David Clark
Dec 26, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Qinghao XuCharles E Inturrisi
Jul 14, 2009·The Journal of Pain : Official Journal of the American Pain Society·Neena K SharmaDouglas E Wright
Mar 23, 2011·Journal of Neuroscience Research·Wenguo FanHongwen He
May 15, 2007·Behavioural Brain Research·De-Yong LiangJ David Clark
May 13, 2014·Cephalalgia : an International Journal of Headache·Nanna ArngrimJes Olesen
Apr 5, 2007·Reviews in the Neurosciences·Johannes Gjerstad
Mar 8, 2008·Pharmacological Reviews·Nader G Abraham, Attallah Kappas

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