Alterations in System xc- Expression in the Retina of Type 1 Diabetic Rats and the Role of Nrf2

Molecular Neurobiology
Raul Carpi-Santos, Karin C Calaza

Abstract

Nrf2 (nuclear factor erythroid 2-related factor 2), a transcription factor that controls expression of several proteins that are related to cellular antioxidant capacity, such as the subunit xCT of the system xc-, is dysregulated in diabetes. Recently, it was described that system xc- is decreased in the retina after 3 weeks of diabetes. So, in the present work, the temporal relationship between xCT and Nrf2 in the retina of diabetic animals was investigated. Diabetes was induced in male Wistar rats (200 g) by a single injection of streptozotocin, and retinas were collected after 1, 2, and 6 months of diabetes induction. Expression of xCT, Nrf2 activity, and binding to antioxidant-responsive element (ARE) sequence were evaluated. Glutathione and reactive oxygen species (ROS) levels were also assessed. After 1 month of diabetes, Nrf2 activity, xCT expression, and glutathione levels were reduced whereas ROS were increased. Although glutathione and ROS levels remain unchanged until later stages, Nrf2 activity and xCT expression returned to normal levels after 2 months. However, they were decreased again at 6 months of diabetes. Accordingly, Nrf2 binding to xCT ARE sequence followed the same pattern of Nrf2 activity and xCT express...Continue Reading

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Citations

May 7, 2020·Frontiers in Neuroscience·Xiaohua Song, Dingxin Long
Jul 14, 2020·Oxidative Medicine and Cellular Longevity·Renita M MartisJulie C Lim
Feb 18, 2021·World Journal of Diabetes·Er-Fei LuoDong Wang
Mar 7, 2021·Neurochemistry International·Jesús Silvestre Albert-GarayRocío Salceda
Jul 27, 2021·Frontiers in Cell and Developmental Biology·Jia-Yue DuanLing-Qing Yuan
Jul 18, 2018·Brain Research·Henrique Rocha MendonçaAna Maria Blanco Martinez

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Methods Mentioned

BETA
ubiquitination
PCR
immunoprecipitation
ChIP
acetylation

Software Mentioned

GraphPad
GraphPad Prism

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