Mar 18, 2020

Alterations in the human plasma lipidome in response to Tularemia vaccination

Kristal Maner-SmithEric A. Ortlund


Tularemia is a highly infectious and contagious disease caused by bacteria Francisella tularensis. To better understand human response to a live, attenuated tularemia vaccine and the biological pathways altered post-vaccination, healthy adults were vaccinated and plasma was collected pre- and post-vaccination for longitudinal lipidomics studies. Using tandem mass spectrometry, we fully characterized individual lipid species within predominant lipid classes to identify changes in the plasma lipidome during the vaccine response. Separately, we targeted oxylipins, a subset of lipid mediators involved in inflammatory pathways. We identified 14 differentially abundant lipid species from eight lipid classes. These included 5-Hydroxyeicosatetraenoic acid (5-HETE) which is indicative of lipoxygenase activity and, subsequently, inflammation. Results suggest that 5-HETE was metabolized to a dihydroxyeicosatrienoic acid (DHET) by Day 7 post-vaccination, shedding light on the kinetics of the 5-HETE-mediated inflammatory response. In addition to 5-HETE and DHET, we observed pronounced changes in 34:1 phosphatidylinositol, anandamide, oleamide, ceramides, 16:1 cholesteryl ester, and other glycerophospholipids; several of these changes in abu...Continue Reading

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Mentioned in this Paper

Arachidonate 5-Lipoxygenase
Dietary Phosphatidylinositol
Communicable Diseases
T-Cell Activation
Tandem Mass Spectrometry
Vaccinated (Qualifier)

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