Mar 18, 2020

Alterations in the human plasma lipidome in response to Tularemia vaccination

bioRxiv
Kristal Maner-SmithEric A. Ortlund

Abstract

Tularemia is a highly infectious and contagious disease caused by bacteria Francisella tularensis. To better understand human response to a live, attenuated tularemia vaccine and the biological pathways altered post-vaccination, healthy adults were vaccinated and plasma was collected pre- and post-vaccination for longitudinal lipidomics studies. Using tandem mass spectrometry, we fully characterized individual lipid species within predominant lipid classes to identify changes in the plasma lipidome during the vaccine response. Separately, we targeted oxylipins, a subset of lipid mediators involved in inflammatory pathways. We identified 14 differentially abundant lipid species from eight lipid classes. These included 5-Hydroxyeicosatetraenoic acid (5-HETE) which is indicative of lipoxygenase activity and, subsequently, inflammation. Results suggest that 5-HETE was metabolized to a dihydroxyeicosatrienoic acid (DHET) by Day 7 post-vaccination, shedding light on the kinetics of the 5-HETE-mediated inflammatory response. In addition to 5-HETE and DHET, we observed pronounced changes in 34:1 phosphatidylinositol, anandamide, oleamide, ceramides, 16:1 cholesteryl ester, and other glycerophospholipids; several of these changes in abu...Continue Reading

  • References
  • Citations

References

  • We're still populating references for this paper, please check back later.
  • References
  • Citations

Citations

  • This paper may not have been cited yet.

Mentioned in this Paper

Arachidonate 5-Lipoxygenase
Lipids
Oleylamide
Dietary Phosphatidylinositol
Serum
Communicable Diseases
T-Cell Activation
Tandem Mass Spectrometry
Species
Vaccinated (Qualifier)

Related Feeds

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.

Related Papers

Monatshefte für Veterinärmedizin
G Heidel
Archives belges de médecine sociale, hygiène, médecine du travail et médecine légale. Belgisch archief van sociale geneeskunde, hygiëne, arbeidsgeneeskunde en gerechtelijke geneeskunde
R BECKERS
The Journal of the Arkansas Medical Society
Annals of Surgery
C C Pinkerton, R H Markwith
© 2020 Meta ULC. All rights reserved