Alterations in the phospholipid composition of Rhodopseudomonas sphaeroides and other bacteria induced by Tris.

Journal of Bacteriology
T J DonohueS Kaplan

Abstract

Alterations in the phospholipid head group composition of most strains of Rhodopseudomonas sphaeroides, as well as Rhodopseudomonas capsulata and Paracoccus denitrificans, occurred when cells were grown in medium supplemented with Tris. Growth of R. sphaeroides M29-5 in Tris-supplemented medium resulted in the accumulation of N-acylphosphatidylserine (NAPS) to as much as 40% of the total whole-cell phospholipid, whereas NAPS represented approximately 28 an 33% of the total phospholipid when R. capsulata and P. denitrificans respectively, were grown in medium containing 20 mM Tris. The accumulation of NAPS occurred primarily at the expense of phosphatidylethanolamine in both whole cells and isolated membranes of R. sphaeroides and had no detectable effect on cell growth under either chemoheterotrophic or photoheterotrophic conditions. Yeast extract (0.1%) and Casamino Acids (1.0%) were found to be antagonistic to the Tris-induced (20 mM) alteration in the phospholipid composition of R. sphaeroides. The wild-type strains R. sphaeroides 2.4.1 and RS2 showed no alteration in their phospholipid composition when they were grown in medium supplemented with Tris. In all strains of Rhodospirillaceae tested, as well as in P. denitrifican...Continue Reading

Citations

Feb 1, 1987·Journal of Bacteriology·R Acosta, D R Lueking
Aug 1, 1983·Journal of Bacteriology·T B Campbell, D R Lueking
Apr 25, 1984·Biochimica Et Biophysica Acta·H H Bodemann, B Karsch
Jan 1, 1990·Progress in Lipid Research·H H SchmidV Natarajan
Jan 1, 1983·Journal of Cellular Biochemistry·S KaplanG S Yen
Mar 4, 2015·Journal of Bacteriology·Kimberly C LemmerTimothy J Donohue

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