Alterations in the production of 14CO2 and [14C]acetylcholine from [U-14C]glucose in brain subregions following transient forebrain ischemia in the rat
Abstract
The production of 14CO2 and [14C )acetylcholine from [U-14C]glucose was determined in vitro using tissue prisms prepared from the dorsolateral striatum (a region developing extensive neuronal loss following ischemia) and the paramedian neocortex (an ischemia-resistant region) following 30 min of forebrain ischemia and recirculation up to 24 h. Measurements were determined under basal conditions (5 mM K+) and following K+ depolarization (31 mM K+). The production of 14CO2 by the dorsolateral striatum was significantly reduced following 30 min of ischemia for measurements in either 5 or 31 mM K+ but recovered toward preischemic control values during the first hour of recirculation. Further recirculation resulted in 14CO2 production again being reduced relative to control values but with larger differences (20-27% reductions) detectable under depolarized conditions at recirculation times up to 6 h. Samples from the paramedian neocortex showed no significant changes from control values at all time points examined. [14C]Acetylcholine synthesis, a marker of cholinergic terminals that is sensitive to changes in glucose metabolism in these structures, was again significantly reduced only in the dorsolateral striatum. However, even in t...Continue Reading
References
Recovery of brain mitochondrial function in the rat after complete and incomplete cerebral ischemia.
Citations
Biochemical changes associated with selective neuronal death following short-term cerebral ischaemia
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