Alterations in the regulatory pathway involving p16, pRb and cdk4 in human chondrosarcoma

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
J AspA Lindahl

Abstract

The G1 regulatory pathway involving p16, pRb and cdk4 in the cell cycle has been investigated in human chondrosarcoma. The protein expression of p16, pRb and cdk4 was analyzed by Western blot in cultured cells from eight chondrosarcomas and in two chondrosarcoma cell lines. Both cell lines and one other sample were negative for p16. Moreover, one of the cell lines was pRb-negative and showed a high expression of cdk4 as well. In the other cell line and in three other samples pRb of expected size were detected in addition to a shorter form of the protein. To further investigate the reasons for down-regulation of the p16 protein, the p16-coding gene CDKN2 was analyzed by polymerase chain reaction (PCR), methyl-specific PCR (MSP) and sequencing in all tumor samples as well as in corresponding tumor tissues from three of the samples. The p16-negative samples were all found to have homozygous deletion of CDKN2. Another sample showed partial gene methylation and a heterozygous position in codon 148 was detected in one sample. The same base substitution was also found in two of the tissue samples. Finally, cytogenetic analysis of the samples with homozygously deleted CDKN2 revealed multiple structural abnormalities in all three cases....Continue Reading

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Citations

Oct 19, 2006·Skeletal Radiology·W C BellG P Siegal
Jan 25, 2014·Cellular Oncology (Dordrecht)·Andre M SamuelDieter M Lindskog
Feb 22, 2012·Pathology Oncology Research : POR·Silvia Calabuig-FariñasAntonio Llombart-Bosch
Jul 15, 2009·Cancer Genetics and Cytogenetics·Meera HameedGokce A Toruner
Jun 8, 2012·Genes, Chromosomes & Cancer·Danielle MeijerJudith V G M Bovée
Jun 13, 2006·Biochemical and Biophysical Research Communications·Farid Moussavi-HaramiJoseph A Buckwalter
Sep 25, 2004·Journal of Cellular Physiology·Frank Beier
Jun 5, 2007·Current Opinion in Oncology·Warren A Chow
Jun 11, 2010·Nature Reviews. Cancer·Judith V M G BovéeBenjamin A Alman
Jun 17, 2003·Laboratory Investigation; a Journal of Technical Methods and Pathology·Rosario Gil-BensoAntonio Llombart-Bosch
May 9, 2014·Ophthalmic Plastic and Reconstructive Surgery·Brett W DaviesVikram D Durairaj
Feb 28, 2019·Cell Communication and Signaling : CCS·Zhengxiao OuyangTang Liu
Jun 3, 2021·Cancers·Agnieszka E ZającPiotr Rutkowski
Jul 16, 2014·Genome Research·Yasushi TotokiTatsuhiro Shibata

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