Alterations in the Rumen Liquid-, Particle- and Epithelium-Associated Microbiota of Dairy Cows during the Transition from a Silage- and Concentrate-Based Ration to Pasture in Spring

Frontiers in Microbiology
M SchärenS Dänicke

Abstract

In spring dairy cows are often gradually transitioned from a silage- and concentrate-based ration (total mixed ration, TMR) to pasture. Rumen microbiota adaptability is a key feature of ruminant survival strategy. However, only little is known on the temporal and spatial microbial alterations involved. This study aims to investigate how the rumen liquid (LAAB), particle (PAAB), and epithelium (EAAB) associated archaea and bacteria are influenced by this nutritional change. A 10-wk trial was performed, including 10 rumen-fistulated dairy cows, equally divided into a pasture- and a confinement- group (PG and CG). The CG stayed on a TMR-based ration, while the PG was gradually transitioned from TMR to pasture (wk 1: TMR-only, wk 2: 3 h/day on pasture, wk 3 & 4: 12 h/day on pasture, wk 5-10: pasture-only). In wk 1, wk 5, and wk 10 samples of solid and liquid rumen contents, and papillae biopsies were collected. The DNA was isolated, and PCR-SSCP and 16S rRNA gene amplicon sequencing analysis were performed. Cluster analysis revealed a higher similarity between LAAB and PAAB, compared to the EAAB, characterized by higher species diversity. At all three locations the microbiota was significantly influenced by the ration change, oppos...Continue Reading

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Citations

Sep 29, 2020·Frontiers in Microbiology·Steven McLoughlinSinéad M Waters
Oct 16, 2018·Frontiers in Microbiology·Roland WirthKornél L Kovács
Dec 29, 2020·Animal Science Journal = Nihon Chikusan Gakkaihō·Toru OgataShigeru Sato
Feb 13, 2021·Microorganisms·Cátia PacíficoQendrim Zebeli
Mar 27, 2021·NPJ Biofilms and Microbiomes·Fabian BillenkampSven Dänicke

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Methods Mentioned

BETA
biopsy
PCR
amplicon sequencing

Software Mentioned

FLASH
vegan
QIIME
cutadapt
lmPerm
Uchime
PERMANOVA
R package vegan
Uclust
R Guide

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