Alterations in tumor necrosis factor-induced endothelial cell procoagulant activity by hyperthermia

International Journal of Cancer. Journal International Du Cancer
Nancy M CarrollH R Alexander

Abstract

TNF is a cytokine with potent antitumor activity in murine models and when administered clinically via regional perfusion. There is substantial evidence that this antitumor activity depends in large part on TNF's procoagulant effect on tumor neovasculature, which is mediated by induction of endothelial cell tissue factor (TF), a component of the extrinsic clotting cascade. In regional perfusion of a cancer-bearing limb or organ, TNF is always administered under hyperthermic temperatures; however, little is known about the effect of hyperthermia on TNF-mediated procoagulant activity in endothelium. We examined the effects of hyperthermia on TNF-mediated procoagulant activity in human umbilical vein endothelial cells (HUVECs). HUVECs were exposed to TNF at normothermic (37 degrees C) and hyperthermic (41 degrees C) temperatures for 90 min, then assayed for clotting activity, TF protein production and mRNA production of TF and tissue factor pathway inhibitor-2 (TFPI-2), an endogenous inducible inhibitor of TF activity in HUVECs. TNF treatment at 41 degrees C significantly reduced clotting activity, TF protein and mRNA as well as TFPI-2 mRNA compared to treatment at 37 degrees C. These data show that hyperthermia significantly redu...Continue Reading

References

Sep 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·E A CarswellB Williamson
Jan 1, 1992·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·D LienardF J Lejeune
Jun 9, 1988·The New England Journal of Medicine·H R MichieD W Wilmore
Nov 3, 1995·The Journal of Biological Chemistry·A BierhausJ Greten
Jul 1, 1995·The Journal of Pathology·N RenardD J Ruiter
Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·C A SprecherD C Foster
Oct 1, 1996·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·A M EggermontF J Lejeune
Oct 1, 1996·Genome Research·C A HeidP M Williams
Apr 21, 1998·Seminars in Surgical Oncology·B C VrouenraetsB B Kroon
Jun 8, 1999·Journal of the American College of Surgeons·R Y Peng, F S Bongard
Jul 27, 1999·Surgical Oncology·J M Hendriks, P E Van Schil
Jan 3, 2001·Journal of Applied Physiology·J D HasdayS E Goldblum
May 16, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·J L FrossardA Hadengue
Jun 22, 2001·European Journal of Surgical Oncology : the Journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·B C VrouenraetsB B Kroon
Jul 26, 2002·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·F MirshahiC Soria
Jan 23, 2003·The Journal of Biological Chemistry·David L BasiMark C Herzberg
Apr 24, 2003·Seminars in Thrombosis and Hemostasis·Naomi LanirBenjamin Brenner

❮ Previous
Next ❯

Citations

Mar 7, 2006·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·X WangJ J Kavanagh
May 9, 2014·International Journal of Cancer. Journal International Du Cancer·Ulrike Leppert, Andreas Eisenreich
Jan 25, 2011·European Journal of Pharmacology·Poyil Pratheeshkumar, Girija Kuttan
Jan 6, 2005·The American Journal of Pathology·Yasunori SatoYasuni Nakanuma
Nov 16, 2010·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Florian GrabellusGeorg Taeger
Oct 30, 2020·International Journal of Biometeorology·Caiyun FanJianbo Cheng

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.