Alterations of 5-hydroxymethylcytosines in circulating cell-free DNA reflect retinopathy in type 2 diabetes.

Genomics
Liyuan HanShiwei Duan

Abstract

Diabetic retinopathy (DR) is a common microvascular complication that may cause severe visual impairment and blindness in patients with type 2 diabetes mellitus (T2DM). Early detection of DR will expand the range of potential treatment options and enable better control of disease progression. Epigenetic dysregulation has been implicated in the pathogenesis of microvascular complications in patients with T2DM. We sought to explore the diagnostic value of 5-hydroxymethylcytosines (5hmC) in circulating cell-free DNA (cfDNA) for DR, taking advantage of a highly sensitive technique, the 5hmC-Seal. The genome-wide 5hmC profiles in cfDNA samples from 35 patients diagnosed with DR and 35 age-, gender-, diabetic duration-matched T2DM controls were obtained using the 5hmC-Seal, followed by a case-control analysis and external validation. The genomic distribution of 5hmC in cfDNA from patients with DR reflected potential gene regulatory relevance, showing co-localization with histone modification marks for active expression (e.g., H3K4me1). A three-gene signature (MESP1, LY6G6D, LINC01556) associated with DR was detected using the elastic net regularization on the multivariable logistic regression model, showing high accuracy to distingui...Continue Reading

References

Sep 6, 2000·Diabetic Medicine : a Journal of the British Diabetic Association·A HutchinsonA Booth
May 23, 2001·Trends in Cardiovascular Medicine·Y SagaS Miyagawa-Tomita
Sep 18, 2003·Ophthalmology·C P WilkinsonUNKNOWN Global Diabetic Retinopathy Project Group
Aug 13, 2005·Bioinformatics·Tobias SingThomas Lengauer
Feb 17, 2010·Journal of Ocular Biology, Diseases, and Informatics·Melinda TeaKeryn A Williams
Oct 15, 2010·Nature Biotechnology·Bradley E BernsteinJames A Thomson
Jan 12, 2011·Nature Biotechnology·James T RobinsonJill P Mesirov
Mar 6, 2012·Nature Methods·Ben Langmead, Steven L Salzberg
Sep 8, 2012·Genome Research·Jennifer HarrowTim J Hubbard
Jan 4, 2013·Diabetes Care·UNKNOWN American Diabetes Association
May 3, 2014·PloS One·Alexey ProninVladlen Z Slepak
Dec 18, 2014·Genome Biology·Michael I LoveSimon Anders
Oct 18, 2015·Nucleic Acids Research·Minoru KanehisaMao Tanabe
Dec 18, 2015·Experimental Eye Research·Sayon RoySumon Roy
Apr 22, 2016·Human Genomics·Chelsea L LoughnerShivalingappa K Swamynathan
Jul 28, 2016·Frontiers in Aging Neuroscience·Isidro FerrerStefano Gustincich
Aug 25, 2016·Diabetes Care·Janet L LeasherUNKNOWN Vision Loss Expert Group of the Global Burden of Disease Study
Feb 9, 2017·Frontiers in Cellular Neuroscience·Nikolina JovancevicLian Gelis
Jun 3, 2017·Vision Research·Heping Xu, Mei Chen
Nov 7, 2017·Diabetic Medicine : a Journal of the British Diabetic Association·H ZaghloulS Taheri
Dec 6, 2018·Journal of Cellular Physiology·Rana ShafabakhshZatollah Asemi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.