Alterations of A549 lung cell gene expression in response to biochemical toxins

Cell Biology and Toxicology
D E BoesewetterM R Riley

Abstract

Health risks associated with the inhalation of potentially toxic materials have been a topic of great public concern. In vitro cellular analyses can provide mechanistic information on the molecular-level responses of lung-derived cell lines to a variety of these hazards. This understanding may be used to develop methods to reduce the damage from such toxins or to detect early stages of their effects. Here we describe an evaluation of the alterations in gene expression of an immortalized lung cell line (A549, human type II epithelia) to a variety of inhalation health hazards including etoposide, gliotoxin, streptolysin O, methyl methansesulfonate (MMS), and Triton X-100. The A549 cells display a dose-response relationship to each toxin with initial responses including alterations in metabolic activity, increases in membrane permeability, and initiation of response genes. In general, membrane-damaging agents (streptolysin O and Triton X-100) induce production of new ion channel proteins, structural proteins, and metabolic enzymes. Gliotoxin impacted the metabolic machinery, but also altered ion channels. Etoposide and MMS caused alterations in the cell cycle, induced DNA repair enzymes, and initiated apoptotic pathways, but MMS a...Continue Reading

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Citations

Apr 15, 2009·Annals of Biomedical Engineering·Georgios PyrgiotakisBrij M Moudgil
Apr 16, 2013·Cell Biology and Toxicology·Josée-Anne Sauvageau, Catherine Jumarie
Jan 28, 2009·Applied Biochemistry and Biotechnology·Dianne E PetersonMark R Riley
Jul 23, 2008·Mutagenesis·Lucy C RichesNigel J Gooderham
Aug 27, 2011·Artificial Intelligence in Medicine·Mario R GuarracinoPanos M Pardalos
Aug 11, 2006·Biotechnology and Bioengineering·Mark R RileyBruno Bureau
Aug 30, 2011·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Marc ManthaCatherine Jumarie

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