Alterations of beta-adrenoceptor responsiveness in postischemic myocardium after 72 h of reperfusion

European Journal of Pharmacology
Matthieu BoucherGuy Rousseau

Abstract

To determine the effect of a completely developed reperfused myocardial infarction model on beta-adrenoceptor responsiveness, we induced a 90-min regional ischemia followed by 72 h of reperfusion in dog hearts. Regional myocardial blood flow was determined after 60 min of ischemia using radioactive microspheres. beta-adrenoceptor density was reduced in the ischemic endocardium (95+/-16 fmol/mg) and epicardium (160+/-13 fmol/mg) compared to the nonischemic region (304+/-21 fmol/mg). beta-adrenoceptor density in the ischemic endocardium varied with the degree of collateral blood flow measured (r2=0.79, P<0.05); this relation was the opposite of that in the ischemic epicardium (r2=0.77, P<0.05). Higher levels of tissue catecholamines and G protein-coupled receptor kinase 2 (GRK2) were observed in the ischemic epicardium as compared to nonischemic tissue. Forskolin-induced adenylyl cyclase activities were depressed in both ischemic regions as compared to nonischemic region, correlating with a reduction in regional myocardial blood flow. Using forskolin stimulation as covariate, no difference in isoproterenol-induced adenylyl cyclase activity was identified in the different regions. It is concluded that cAMP production induced by be...Continue Reading

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Citations

Jan 1, 2009·Cardiovascular Psychiatry and Neurology·Mark E ObrenovichGjumrakch Aliev
Mar 19, 2009·Journal of Cellular and Molecular Medicine·Mark E ObrenovichGjumrakch Aliev
Oct 7, 2005·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Xiurong ZhaoJaroslaw Aronowski
Aug 8, 2019·International Journal of Molecular Sciences·Louise E See HoeJohn F Fraser
Apr 28, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Matthew A WellsUNKNOWN Dead Heart Project
May 1, 2021·Cells·Anne-Sophie Colombe, Guillaume Pidoux

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