PMID: 8589032Nov 1, 1995Paper

Alterations of CDKN2 (p16) in non-small cell lung cancer

Genes, Chromosomes & Cancer
S de VosH P Koeffler

Abstract

The cyclin-dependent kinase inhibitor known as p16 (CDK41, CDKN2, INK4A, MTS1) has been proposed as a tumor suppressor gene on chromosome segment 9p21. We have evaluated CDKN2 alterations in 34 non-small cell lung cancers (NSCLCs) with matched normal tissue controls and in 9 NSCLC cell lines by Southern blotting, single-strand conformation polymorphism (SSCP) with the polymerase chain reaction, and direct sequencing. In addition, loss of heterozygosity at chromosome segment 9p21, with the use of the microsatellite marker D9S171, was studied in these samples. Whereas CDKN2 was either deleted or mutated in NSCLC cell lines at a high frequency (6/9, 67%), alterations were much less frequent (7/34, 21%) in primary tumor samples. Only one sample contained a point mutation in exon 1 of CDKN2. In addition, two samples had homozygous deletions of CDKN2 in exon 1; one had a homozygous and three a hemizygous deletion of exon 2. Possibly normal tissue contaminating our tumor samples may have masked homozygous deletions in these cases. Four patient samples had LOH in the region of CDKN2 on chromosome segment 9p21; two of these samples had potentially inactivating alterations of CDKN2; one sample had a mutation of CDKN2, and the other had a...Continue Reading

References

Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J W FountainJ Lahti
May 1, 1991·Genes, Chromosomes & Cancer·J Whang-PengJ D Minna
Oct 27, 1989·Science·T TakahashiJ D Minna
Apr 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·A G Knudson
Jul 15, 1994·Science·P CairnsD Sidransky
Jul 21, 1994·Nature·C H SpruckP A Jones
Jun 7, 1994·Proceedings of the National Academy of Sciences of the United States of America·L HengstS I Reed
Nov 19, 1993·Cell·W S el-DeiryB Vogelstein
Dec 3, 1993·Cell·T Hunter
Dec 16, 1993·Nature·Y XiongD Beach
Jan 18, 1994·Proceedings of the National Academy of Sciences of the United States of America·P W HindsR A Weinberg
Jun 18, 1993·Cell·C J Sherr

❮ Previous
Next ❯

Citations

Mar 12, 2004·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·A DestroM Roncalli
May 1, 1997·Cancer Genetics and Cytogenetics·J R TestaT Taguchi
Sep 18, 1998·Biochimica Et Biophysica Acta·Y SekidoJ D Minna
Jul 24, 2008·Toxicologic Pathology·Hue-Hua L HongStephanie A Lahousse
Jun 30, 2005·BMC Gastroenterology·Jyotika AttriJaidev Wig
Jul 5, 2003·Journal of Tongji Medical University = Tong Ji Yi Ke Da Xue Xue Bao·S ZhuH Wu
Jan 1, 1997·Environmental and Molecular Mutagenesis·L PylkkänenK Husgafvel-Pursiainen
Apr 10, 1997·International Journal of Cancer. Journal International Du Cancer·L J MeadL J Campbell
Feb 14, 1998·International Journal of Cancer. Journal International Du Cancer·A MarchettiG Bevilacqua
Nov 6, 2002·Japanese Journal of Cancer Research : Gann·Naoki YanagawaTeiichi Motoyama
Jul 5, 2005·Expert Opinion on Investigational Drugs·K R Webster
Oct 1, 1996·Leukemia & Lymphoma·M Heyman, S Einhorn
May 28, 2013·The International Journal of Biological Markers·Ping ChenXing-Ping Tong
Mar 17, 2004·Oncogene·Sandra W LeungLili Yamasaki
Nov 21, 1998·Biochimica Et Biophysica Acta·M Ruas, G Peters

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.