Alterations of concentrations of calcium and arachidonic acid and agglutinations of microfilaments in host cells during Toxoplasma gondii invasion

Veterinary Parasitology
Liwei LiJie Yan

Abstract

Toxoplasma gondii (T. gondii) invasion of host cells is a complicated process of interaction between parasites and host cells. In the present study we investigated the alterations of free Ca2+ concentration ([Ca2+]i) and cytoskeletons in phagocytic and non-phagocytic host cells and arachidonic acid (AA) concentration in cells supernatant during T. gondii invasion. T. gondii invasion induced significant elevation of intracellular [Ca2+]i in phagocytic cells (J774A.1) but not in non-phagocytic cells (L929). Pre-treatment of J774A.1 cells with Phospholipase C (PLC) inhibitor (U73122), or Ca2+ chelators (EGTA, BAPTA/AM) did not block elevations of [Ca2+]i but the elevations were lower and of shorter duration than that in untreated cells. Pre-treatment of tachyzoites with Phospholipases A (PLA) inhibitors (4-BPB and AACOCF3) resulted in a similar pattern of increasing of [Ca2+]i as that in Ca2+ chelators treated cells. Agglutinations of microfilaments were observed in J774A.1 cells but not in L929 cells. No changes of microtubules were observed in either cell. Treatment of cells with cytoskeleton inhibitors (colchicines, cytochalasin-D) resulted in reduced cell infection ratios. AA concentration in J774A.1 cells supernatant reached ...Continue Reading

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Citations

Dec 29, 2011·Functional & Integrative Genomics·Robert W Li, Steven G Schroeder
Jul 3, 2013·Microbes and Infection·Abdur RubYusuf Akhter
Nov 16, 2013·BioMed Research International·José Luis VegaJuan Carlos Sáez
Jun 21, 2018·Frontiers in Cellular and Infection Microbiology·Xiao-Qing ChenXing-Quan Zhu

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