Alterations of FSH-stimulated progesterone production and calcium homeostasis in primarily cultured human luteinizing-granulosa cells induced by fenvalerate

Toxicology
Jun HeXin-Ru Wang

Abstract

Fenvalerate, a synthetic pyrethroid, is widely used in agriculture and other domestic applications in China. Recently, Fenvalerate has been suspected to be one of the endocrine-disrupting chemicals (EDC). In this study, we investigated the effects of fenvalerate on follicle-stimulating hormone (FSH)-stimulated progesterone (P4) production by human ovarian luteinizing-granulosa cells (hGLCs). After 24 h incubation, fenvalerate inhibited FSH-stimulated P4 production. At the same time, FSH-stimulated cAMP also decreased. Due to calcium and Ca2+ -calmodulin (CaM) system involving gonadotropin-stimulated steroidogenesis by granulosa cells, we then evaluated the effects of fenvalerate on trifluoperazine (TFP)- and verapamil-driven FSH-stimulated P4 production. The results showed that calcium or calmodulin might play a role in fenvalerate-induced alterations in FSH-stimulated P4 biosynthesis. Then, the effects of fenvalerate on calcium homeostasis in hGLCs were studied. The result showed that 5 microM fenvalerate induced a slow increase in [Ca2+]i in hGLCs by using a fluorescent Ca2+ indicator fluo-3/AM. The changes in total concentration of CaM in hGLCs induced by fenvalerate were evaluated by a method of immunofluorescence. There is...Continue Reading

References

Sep 21, 1990·Biochimica Et Biophysica Acta·F MichelangeliA G Lee
Dec 1, 1994·Endocrine Reviews·J S Richards
Feb 1, 1993·Endocrine Reviews·K P Lu, A R Means
Oct 29, 1998·Biochemical and Biophysical Research Communications·J Garey, M S Wolff
Oct 25, 2002·Journal of Toxicology and Environmental Health. Part a·Haiyan ChenXinru Wang
Jul 2, 2003·Toxicological Sciences : an Official Journal of the Society of Toxicology·Terri Damstra

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Citations

Sep 4, 2009·Biological Trace Element Research·Belma GirayFiliz Hincal
May 12, 2009·Reproductive Toxicology·John D MeekerRuss Hauser
Nov 14, 2008·Fertility and Sterility·Hsien-An PanPao-Lin Kuo
Dec 7, 2007·Toxicology Letters·Jian-Hua QuXin-Ru Wang
Mar 3, 2007·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Xia HongXinru Wang
Jul 12, 2005·Neurotoxicology and Teratology·A C MonizM M Bernardi
Oct 17, 2008·Journal of Toxicology and Environmental Health. Part a·Arielle C ArenaWilma G Kempinas
Jan 18, 2011·Human & Experimental Toxicology·Belma Giray, Filiz Hincal

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