PMID: 8612613Mar 1, 1996Paper

Alterations of lipoprotein fluidity by non-esterified fatty acids known to affect cholesteryl ester transfer protein activity. An electron spin resonance study

European Journal of Biochemistry
C FoucherP Gambert

Abstract

The aim of the present study was to investigate the effect of saturated, monounsaturated and polyunsaturated non-esterified fatty acids (NEFA) on lipoprotein fluidity by using the electron spin resonance (ESR) method. The fluidity of the lipid phase of lipoproteins was evaluated by calculating from ESR spectra the S parameter of three different positional isomers of spin-labeled stearic acid incorporated into the lipoprotein. In non-enriched lipoproteins, S values were higher in high-density lipoprotein 3 (HDL3) than in low-density lipoprotein (LDL) indicating that the surface of HDL3 was more ordered. Prior incubation of lipoprotein particles with NEFA significantly reduced S values, indicating an increased lipoprotein fluidity as compared with non-supplemented homologous samples. In NEFA-enriched lipoproteins, the modifications in fluidity were shown to be dependent on the structure of the NEFA acyl carbon chains. Medium-chain fatty acids [lauric (12:0) and myristic (14:0) acids] appeared to be better fluidizing molecules as compared with both shorter [octanoic (8:0) and decanoic (10:0) acids] and longer [palmitic (16:0) and stearic (18:0) acids] homologues. In addition, introducing at least one double bond in the acyl carbon...Continue Reading

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Citations

Sep 18, 2008·European Biophysics Journal : EBJ·Ruth Prassl, Peter Laggner
Dec 8, 2009·The Journal of Nutritional Biochemistry·Javier S PeronaValentina Ruiz-Gutierrez
Jul 25, 2000·Journal of Pharmaceutical Sciences·O ChambinY Pourcelot
Apr 26, 2000·Prostaglandins, Leukotrienes, and Essential Fatty Acids·V Manjari, U N Das

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