Alterations of mitochondrial electron transport chain and oxidative stress induced by alkaloid-like α-aminonitriles on Aedes aegypti larvae

Pesticide Biochemistry and Physiology
Mayra A Borrero LandazabalStelia C Mendez-Sanchez

Abstract

Aedes aegypti mosquitoes are responsible for dengue, chikungunya, and Zika virus transmission in tropical and subtropical areas around the world. Due to the absence of vaccines or antiviral drugs for human treatment, the majority of control strategies are targeted at Ae. aegypti elimination. Our research on mosquito control insecticidal agents has previously shown that the alkaloid girgensohnine and its analogues (α-aminonitriles) present in vitro acetylcholinesterase inhibition and in vivo insecticidal activity against Ae. aegypti. However, acetylcholinesterase inhibition may not be the only mechanism of action behind these effects. On this basis, the principal aim of this study was to elucidate the possible action mode of four α-aminonitriles on Ae. aegypti by studying other important enzymatic targets, such as mitochondrial electron transport chain complexes, catalase, and superoxide dismutase, key oxidative stress enzymes. Mitochondria were isolated from Ae. aegypti larvae by differential centrifugation, stored at -70°C, and fragmented using ultrasound for 10min. The effects of α-aminonitriles (1 to 4) over enzymatic activities were evaluated using concentrations of 8nM, 2μM, 8μM, and 40μM. Results indicated that α-aminonit...Continue Reading

Citations

Nov 11, 2019·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Mayra A Borrero-LandazabalStelia C Mendez-Sanchez
Jun 25, 2020·Mini Reviews in Medicinal Chemistry·Maria Beatriz Silva Costa, Camila Martins de Oliveira
Dec 17, 2021·Future Microbiology·Chandan SarkarNatália Cruz-Martins

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