PMID: 3768422Nov 19, 1986Paper

Alterations of purine salvage pathways during differentiation of rat heart myoblasts towards myocytes

Biochimica Et Biophysica Acta
G SchopfM M Müller

Abstract

Enzyme activities of purine catabolism and salvage, the concentrations of high-energy phosphates and the reutilisation of purine bases and purine nucleosides were studied in rat heart myoblasts and myocytes. Rat heart myoblasts H9c2(2-1) were grown in Dulbecco's modified Eagle's minimum essential medium supplemented with 10% fetal calf serum. Reduction of fetal calf serum to 2% for 1 week resulted in a differentiation into myocytes with respect to their morphological features and their enzyme pattern. In differentiated myocytes, activity of 5'-nucleotidase was increased more than 2-fold, and AMP deaminase and creatine kinase activities were more than 10-fold elevated. The concentration of creatine phosphate in differentiated myocytes was doubled compared to that in myoblasts. The uptake into myoblasts and myocytes and the incorporation into adenine nucleotides was highest using adenosine, inosine and adenine uptake rates were intermediate, and hypoxanthine was utilised least. Differentiation of myoblasts into myocytes resulted in a slightly lower overall uptake of adenosine and adenine, whereas about 40% more inosine and hypoxanthine were utilised by myocytes. Increasing the phosphate concentration in the incubation medium up t...Continue Reading

References

Sep 29, 1978·Pflügers Archiv : European journal of physiology·H G Zimmer, E Gerlach
Mar 15, 1976·Experimental Cell Research·B W Kimes, B L Brandt
Jan 1, 1985·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·H Stone, J Overnell
Jan 1, 1985·Annual Review of Physiology·J P Manfredi, E W Holmes
Jan 1, 1986·Advances in Experimental Medicine and Biology·M M MüllerP Zilla
Jan 1, 1986·Advances in Experimental Medicine and Biology·G SchopfM M Müller
Jan 1, 1985·The American Journal of Physiology·M J HollandJ K Kelleher
Feb 21, 1985·Biochimica Et Biophysica Acta·J BowditchJ W Dow
Mar 1, 1985·Basic Research in Cardiology·I Leprán, A M Lefer
Nov 1, 1972·Circulation Research·R A OlssonG P Frick
Mar 14, 1972·Biochimica Et Biophysica Acta·M H MaguireJ F Rettie
Jan 1, 1983·Advances in Enzyme Regulation·M M MüllerH Rumpold
Jun 16, 1982·Biochimica Et Biophysica Acta·E Zoref-ShaniO Sperling
Oct 31, 1983·Biochemical and Biophysical Research Communications·E Zoref-ShaniO Sperling
Feb 15, 1983·Biochimica Et Biophysica Acta·J M LamersN S Dhalla
Jan 1, 1984·The American Journal of Physiology·P MauduitB Rossignol
Jun 19, 1984·Biochimica Et Biophysica Acta·E Zoref-ShaniO Sperling

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Citations

Jun 30, 2012·Cardiovascular Toxicology·Ana F BrancoVilma A Sardão
Dec 1, 1993·Free Radical Biology & Medicine·A GriesmacherM M Mueller
Feb 25, 2003·Progress in Retinal and Eye Research·Gerard A Lutty, D Scott McLeod
Jun 4, 2013·Biochemical and Biophysical Research Communications·Daigo SumiSeiichiro Himeno
Aug 13, 2005·The Journal of Biological Chemistry·Michal ArmoniEddy Karnieli
Dec 1, 1995·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·E Zoref-ShaniO Sperling
Feb 13, 1993·Biochimica Et Biophysica Acta·P RotllanP Llorente
Jun 30, 1988·Biochimica Et Biophysica Acta·A E JacobsJ H Veerkamp
Jul 11, 2006·International Journal of Cardiology·George P H LeungRicky Y K Man
Aug 17, 2021·Frontiers in Cardiovascular Medicine·Hao Yi-DanZhou Yu-Jie

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