Alterations of Spermatozoa Proteomic Profile in Men with Hodgkin's Disease Prior to Cancer Therapy

The World Journal of Men's Health
Ana Dias MartinsManesh Kumar Panner Selvam

Abstract

Hodgkin's disease (HD) is a type of cancer affecting men in the reproductive age with potential consequences on their fertility status. This study aims to analyze sperm parameters, alterations in proteomic profiles and validate selected protein biomarkers of spermatozoa in men with HD undergoing sperm banking before cancer therapy. Semen analysis was carried out in healthy fertile donors (control, n=42), and patients diagnosed with HD (patients, n=38) before cancer therapy. We compared proteomic profiles of spermatozoa from donors (n=3) and patients (n=3) using LTQ-Orbitrap Elite hybrid MS system. A total of 1,169 proteins were identified by global proteomic in both groups. The ingenuity pathway analysis revealed that differentially expressed proteins involved in capacitation, acrosome reaction, binding of sperm to the zona pellucida, sperm motility, regulation of sperm DNA damage, and apoptosis were significantly downregulated in HD patients. Validation of proteins implicated in sperm fertility potential by Western Blot demonstrated that peroxiredoxin 2 (PRDX 2) was underexpressed (p=0.015), and transferrin (p=0.045) and SERPIN A5 (p=0.010) protein levels were overexpressed in spermatozoa of men with HD. Findings of this study...Continue Reading

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Citations

Sep 19, 2020·International Journal of Molecular Sciences·Manesh Kumar Panner SelvamAshok Agarwal

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Methods Mentioned

BETA
proteomic profiling
electrophoresis
protein folding

Software Mentioned

Ingenuity Pathways Analysis ( IPA )
MedCalc Statistical
! Tandem
GO Term Finder
Proteome Discoverer
GO Mapper
Proteome
CYCLONE

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