PMID: 2499675Jun 1, 1989Paper

Alterations of spontaneous and evoked release of acetylcholine during dithiobiuret-induced neuromuscular weakness

The Journal of Pharmacology and Experimental Therapeutics
W D Atchison

Abstract

Daily treatment of rats with 2,4-dithiobiuret (DTB, 1 mg/kg/day i.p.) produces a flaccid neuromuscular weakness first observed in the hindlimbs after 5 to 6 days of treatment. This condition is characterized by diminished contractile strength following single shock and tetanic stimulation of the motor nerve, but no effect on contractions evoked by direct muscle stimulation, indicating an apparent impairment of motor axon conduction, junctional transmission or both. The purpose of the present study was to investigate further the neuromuscular depression caused by DTB using conventional intracellular microelectrode recording techniques. All experiments were conducted using the extensor digitorum longus muscle isolated from male rats treated for 6 to 7 days with 1 mg/kg/day i.p. of DTB or with 0.9% NaCl (1 ml/kg/day) as control. End-plate potentials (EPPs) and miniature end-plate potentials (MEPPs) were recorded from single junctions of DTB-poisoned or NaCl-treated paired controls. Muscles were transected ("cut muscle") to prevent contraction after peroneal nerve stimulation. EPP amplitude was decreased at the time of observable muscle weakness in DTB-treated rats. Endplate resting membrane potential was not affected. Decreased EP...Continue Reading

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