Alterations of the glucose metabolism in a triose phosphate isomerase-negative Saccharomyces cerevisiae mutant

Yeast
C CompagnoD Porro

Abstract

The absence of triose phosphate isomerase activity causes an accumulation of only one of the two trioses, dihydroxyacetone phosphate, and this produces a shift in the final product of glucose catabolism from ethanol to glycerol (Compagno et al., 1996). Alterations of glucose metabolism imposed by the deletion of the TPI1 gene in Saccharomyces cerevisiae were studied in batch and continuous cultures. The Deltatpi1 null mutant was unable to grow on glucose as the sole carbon source. The addition of ethanol or acetate in media containing glucose, but also raffinose or galactose, relieved this effect in batch cultivation, suggesting that the Crabtree effect is not the primary cause for the mutant's impaired growth on glucose. The addition of an energy source like formic acid restored glucose utilization, suggesting that a NADH/energy shortage in the Deltatpi1 mutant could be a cause of the impaired growth on glucose. The amount of glycerol production in the Deltatpi1 mutant could represent a good indicator of the fraction of carbon source channelled through glycolysis. Data obtained in continuous cultures on mixed substrates indicated that different contributions of glycolysis and gluconeogenesis, as well as of the HMP pathway, to ...Continue Reading

References

Jul 1, 1979·Journal of Bacteriology·M Ciriacy, I Breitenbach
Feb 1, 1989·Applied and Environmental Microbiology·E PostmaJ P Van Dijken
Jan 1, 1984·Annual Review of Microbiology·R A Cooper
May 11, 1999·FEMS Microbiology Letters·M T FlikweertJ T Pronk

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Citations

Jun 26, 2012·Applied and Environmental Microbiology·C VarelaP J Chambers
Jun 1, 2002·Applied and Environmental Microbiology·Karin M OverkampJack T Pronk
Aug 30, 2002·Applied and Environmental Microbiology·Garth R CronwrightBernard A Prior
Nov 2, 2014·Applied Microbiology and Biotechnology·Waleed Ahmad KhattakJoong Kon Park
Jan 14, 2017·Microbial Biotechnology·Hugh D GooldIsak S Pretorius
Sep 26, 2001·Yeast
Dec 20, 2011·Biotechnology and Bioengineering·Zihe LiuJens Nielsen
Nov 1, 2008·The Journal of Microbiology·Hoon Park, Yoon-Sun Hwang
Apr 17, 2010·Applied Microbiology and Biotechnology·Tomohiko MatsuzawaKaoru Takegawa
Feb 11, 2020·Klinicheskaia laboratornaia diagnostika·N A Kolotyeva, F N Gilmiyarova
Aug 23, 2020·Genes·Mikhail A Eldarov, Andrey V Mardanov
Mar 11, 2017·Applied Microbiology and Biotechnology·Marta V SemkivAndriy A Sibirny
Jan 21, 2012·Journal of Food Science·Leigh M SchmidtkeSamson O Agboola
Aug 31, 2017·Scientific Reports·Nurliyana RuzlanHarikrishna Kulaveerasingam

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