PMID: 11920642Mar 29, 2002Paper

Alterations of the p16(ink4a) gene in resected nonsmall cell lung tumors and exfoliated cells within sputum

International Journal of Cancer. Journal International Du Cancer
Jung-Ta ChenYi-Ching Wang

Abstract

Recently, we reported that p16 protein expression was nondetectable in 49.5% of 107 resected nonsmall cell lung cancers (NSCLCs), suggesting that the p16(INK4a) gene is frequently inactivated in primary NSCLC. To identify the molecular basis for this p16 immunohistochemical negativity further, we performed a genetic and epigenetic study of p16(INK4a) status in a series of 115 NSCLC samples parallel to the clinicopathologic and prognostic analyses. Microdissected tumor DNA samples were screened for homozygous deletion using comparative multiplex-polymerase chain reaction (PCR), for intragenic mutation using direct sequencing and for loss of heterozygosity (LOH) using an intragenic microsatellite marker, D9S942. Of these samples, 67 were further analyzed by SmaI-based PCR methylation assay to evaluate aberrant methylation at the gene. To examine the correlation of aberrant methylation in tumor and sputum samples, sputum samples from 12 matched patients were assessed for this change. We found that methylation of the p16(INK4a) gene was present in 38 of the 67 (56.7%) tumors and was significantly associated with negative p16 protein expression (p = 0.029). A 92% (11/12) concordance of sputum samples with matched resected tumors was...Continue Reading

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Citations

Aug 11, 2012·Rejuvenation Research·Giovanna E CarpagnanoMaria P Foschino-Barbaro
Nov 6, 2003·Journal of Clinical Pathology·F B J M Thunnissen
Jun 11, 2005·American Journal of Respiratory and Critical Care Medicine·Giovanna E CarpagnanoAngelo Paradiso
Oct 27, 2007·American Journal of Respiratory and Critical Care Medicine·Giovanna E CarpagnanoAngelo Paradiso
Jun 28, 2013·PloS One·Xiang-Bin XingMin-Hu Chen
Nov 17, 2006·Annals of the New York Academy of Sciences·Yi-Ching WangJung-Ta Chen
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May 2, 2003·Annals of the New York Academy of Sciences·Arti PatelAsad Umar
Jul 23, 2013·British Journal of Cancer·A J HubersE Thunnissen
Oct 1, 2011·European Journal of Clinical Investigation·Giovanna E CarpagnanoMaria P Foschino-Barbaro
Aug 24, 2013·Expert Review of Respiratory Medicine·Michael FleischhackerBernd Schmidt
May 19, 2005·International Journal of Cancer. Journal International Du Cancer·Ruo-Chia TsengYi-Ching Wang
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Dec 4, 2004·International Journal of Cancer. Journal International Du Cancer·Kevin K DivineSteven A Belinsky

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