PMID: 2106348Feb 23, 1990Paper

Altered arachidonic acid metabolism during differentiation of the human monoblastoid cell line U937

Biochimica Et Biophysica Acta
L KoehlerM Goppelt-Struebe

Abstract

The human cell line U937 was used as a model for differentiation along the mononuclear phagocyte lineage. Following treatment with the phorbol ester TPA, PGE2 and TxB2 secretion was induced 50-100-fold, and both PGF2 alpha and PGI2 levels became detectable in the supernatant of TPA-differentiated U937 cells. The content of the prostaglandin precursor, arachidonic acid, remained unchanged in the cellular phospholipids of undifferentiated and TPA-differentiated U937 cells. Of the enzymes involved in the availability and metabolism of arachidonic acid, phospholipase A2 activity was increased 2-fold in the membranes of TPA-differentiated U937 cells, whereas lysophosphatide acyltransferase activity remained unaltered. Cyclooxygenase activity, however, was enhanced 5-10-fold, which was due to enhanced expression of the enzyme as demonstrated by dot-blot analysis. The data suggest that the capacity to secrete prostaglandins is acquired during differentiation with TPA and results mainly from an increased cyclooxygenase activity. Despite the capacity of TPA-differentiated U937 cells to synthesize prostaglandins, none of the known monocytic stimuli further stimulated prostaglandin secretion in TPA-differentiated U937 cells. Generation of...Continue Reading

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Citations

Apr 24, 1991·Biochimica Et Biophysica Acta·W L Smith, L J Marnett
Jan 1, 1991·Pharmacology & Therapeutics·W L SmithD L DeWitt
Jul 1, 1996·Critical Reviews in Oncology/hematology·C Denzlinger
Jul 5, 2003·Biochimica Et Biophysica Acta·Yan J JiangPatrick C Choy
Nov 1, 1994·British Journal of Pharmacology·A TomlinsonD A Willoughby
Nov 1, 1991·Prostaglandins, Leukotrienes, and Essential Fatty Acids·T HidakaR Ogura
Mar 16, 2013·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Chih-Chiang ChienYen-Chou Chen
Mar 3, 2006·Biochemical and Biophysical Research Communications·Shashi BalaManjit Hanspal
May 8, 1991·Biochimica Et Biophysica Acta·D L DeWitt
Mar 1, 1995·Cell Biochemistry and Function·F C AepfelbacherM Aepfelbacher
Apr 2, 2009·Biological Chemistry·Ralf Hass
Sep 10, 1998·Biochemical and Biophysical Research Communications·M V GoncharA T Mevkh
Jun 8, 2002·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Maria Teresa Rizzo
Feb 5, 1991·Biochimica Et Biophysica Acta·J D WinklerF H Chilton
Oct 1, 1993·Prostaglandins, Leukotrienes, and Essential Fatty Acids·C B EvansM E Goldyne

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