Altered beta-adrenergic regulation of Na-K-Cl cotransport in cultured smooth muscle cells from the aorta of spontaneously hypertensive rats. Role of the cytoskeleton network

American Journal of Hypertension
S N OrlovP Hamet

Abstract

To verify the hypothesis of Na-K-Cl cotransport (COTR) involvement in ion transport abnormalities as revealed in vascular smooth muscle cells (VSMC) of spontaneously hypertensive rats (SHR), we compared the rate of ouabain-insensitive, bumetanide-inhibited 86Rb influx in quiescent and growing cultures of VSMC from the aorta of SHR and normotensive (BN.lx) rats and its regulation by the cAMP signaling system. Basal COTR was not altered in quiescent cells from SHR but was decreased by 30% to 40% (P < .02) in growing SHR VSMC as compared to BN.lx rats. In quiescent BN.lx VSMC, isoproterenol inhibited COTR by 50% and induced cell shape transition of > 90% of cells, resulting in the appearance of rounded VSMC with arborized cytoplasms. In contrast, isoproterenol elicited cell shape transition in only 50% of quiescent SHR VSMC and did not modify COTR. In growing cells, it decreased COTR by 85% to 95% and altered cell morphology in > 95% of VSMC without differences between SHR and BN.lx rats. Neither inhibitors of protein kinase A (H-89 and KT-5720) nor an inhibitor of phosphoprotein phosphatase (okadaic acid) affected cell shape transition and COTR suppression in isoproterenol-treated VSMC. The COTR suppression and cytoplasm arboriza...Continue Reading

Citations

Jun 18, 2003·Muscle & Nerve·Ingrid ToftTrond Jenssen
Jan 23, 2008·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Svetlana V KoltsovaSergei N Orlov
Jul 17, 1999·The American Journal of Physiology·J A WongD B Thomason
Nov 1, 2003·American Journal of Physiology. Cell Physiology·Aidar R GosmanovDonald B Thomason
Mar 10, 1999·The American Journal of Physiology·S N OrlovP Hamet
Feb 28, 2001·Progress in Biophysics and Molecular Biology·A R Chipperfield, A A Harper
Jul 19, 2005·Pathophysiology : the Official Journal of the International Society for Pathophysiology·Olga A AkimovaSergei N Orlov

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