Altered branching patterns of Purkinje cells in mouse model for cortical development disorder.

Scientific Reports
Jinkyung KimIm Joo Rhyu

Abstract

Disrupted cortical cytoarchitecture in cerebellum is a typical pathology in reeler. Particularly interesting are structural problems at the cellular level: dendritic morphology has important functional implication in signal processing. Here we describe a combinatorial imaging method of synchrotron X-ray microtomography with Golgi staining, which can deliver 3-dimensional(3-D) micro-architectures of Purkinje cell(PC) dendrites, and give access to quantitative information in 3-D geometry. In reeler, we visualized in 3-D geometry the shape alterations of planar PC dendrites (i.e., abnormal 3-D arborization). Despite these alterations, the 3-D quantitative analysis of the branching patterns showed no significant changes of the 77 ± 8° branch angle, whereas the branch segment length strongly increased with large fluctuations, comparing to control. The 3-D fractal dimension of the PCs decreased from 1.723 to 1.254, indicating a significant reduction of dendritic complexity. This study provides insights into etiologies and further potential treatment options for lissencephaly and various neurodevelopmental disorders.

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Citations

Feb 27, 2013·Proceedings of the National Academy of Sciences of the United States of America·Lorenzo MagrassiFerdinando Rossi
Oct 2, 2012·The Review of Scientific Instruments·J W JungJ H Je
Jan 15, 2014·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·Claudia CastagnaLaura Lossi
Jun 24, 2015·Trends in Neurosciences·Pamela ValnegriAzad Bonni
May 16, 2014·Brain Structure & Function·Jinkyung KimJung Ho Je
Jun 29, 2012·Scientific Reports·Jinkyung KimJung Ho Je
Mar 5, 2015·Scientific Reports·Soeun ChangAkira Tsuda
Dec 19, 2012·Scientific Reports·Jinkyung KimIm Joo Rhyu
Jul 25, 2017·Cellular and Molecular Life Sciences : CMLS·Fernanda Ledda, Gustavo Paratcha
Jan 29, 2021·Scientific Reports·Julian H SmithR P Taylor

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Methods Mentioned

BETA
light
electron
X-ray

Software Mentioned

Amira5
Matlab

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