Altered calcium dynamics mediates P19-derived neuron-like cell responses to millimeter-wave radiation

Radiation Research
Igor TitushkinM R Cho

Abstract

Intracellular calcium oscillations have long been recognized as a principal mediator of many vital cellular activities. Furthermore, Ca(2+) dynamics can be modulated by external physical cues, including electromagnetic fields. While cellular responses to low-frequency electric fields have been established, the possible non-thermal effects of millimeter-wave (MMW) radiation are still a subject of discussion and debate. We used mouse embryonic stem cell-derived neuronal cells and a custom-built 94 GHz applicator to examine in real time the altered Ca(2+) oscillations associated with MMW stimulation. MMW irradiation at 18.6 kW/m(2) nominal power density significantly increased the Ca(2+) spiking frequency in the cells exhibiting Ca(2+) activity. The N-type calcium channels, phospholipase C enzyme, and actin cytoskeleton appear to be involved in mediating increased Ca(2+) spiking. Reorganization of the actin microfilaments by a 94 GHz field seems to play a crucial role in modulating not only Ca(2+) activity but also cell biomechanics. Many but not all observed cellular responses to MMW were similar to thermally induced effects. For example, cell exposure to a 94 GHz field induced nitric oxide production in some morphologically dist...Continue Reading

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Citations

Oct 20, 2012·Bioelectromagnetics·Andrey Samsonov, Sergey V Popov
Sep 22, 2019·International Journal of Environmental Research and Public Health·Myrtill Simkó, Mats-Olof Mattsson

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Methods Mentioned

BETA
thermal treatment
AFM

Software Mentioned

COMSOL

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