Altered composition of liver proteasome assemblies contributes to enhanced proteasome activity in the exceptionally long-lived naked mole-rat.

PloS One
Karl A RodriguezRochelle Buffenstein

Abstract

The longest-lived rodent, the naked mole-rat (Bathyergidae; Heterocephalus glaber), maintains robust health for at least 75% of its 32 year lifespan, suggesting that the decline in genomic integrity or protein homeostasis routinely observed during aging, is either attenuated or delayed in this extraordinarily long-lived species. The ubiquitin proteasome system (UPS) plays an integral role in protein homeostasis by degrading oxidatively-damaged and misfolded proteins. In this study, we examined proteasome activity in naked mole-rats and mice in whole liver lysates as well as three subcellular fractions to probe the mechanisms behind the apparently enhanced effectiveness of UPS. We found that when compared with mouse samples, naked mole-rats had significantly higher chymotrypsin-like (ChT-L) activity and a two-fold increase in trypsin-like (T-L) in both whole lysates as well as cytosolic fractions. Native gel electrophoresis of the whole tissue lysates showed that the 20S proteasome was more active in the longer-lived species and that 26S proteasome was both more active and more populous. Western blot analyses revealed that both 19S subunits and immunoproteasome catalytic subunits are present in greater amounts in the naked mole-...Continue Reading

References

Jan 1, 1993·Enzyme & Protein·K B HendilP Kristensen
Jan 4, 1994·Proceedings of the National Academy of Sciences of the United States of America·S D PatelH O McDevitt
Jul 8, 1997·Proceedings of the National Academy of Sciences of the United States of America·C S Arendt, M Hochstrasser
Apr 28, 1999·Free Radical Biology & Medicine·P KapahiT B Kirkwood
Jun 8, 1999·Annual Review of Immunology·K L Rock, A L Goldberg
Dec 3, 1999·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·P ZwicklW Baumeister
Jan 29, 2000·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·M Yanagida
Oct 18, 2000·Biochemical and Biophysical Research Communications·C NodaK Tanaka
Nov 4, 2000·Journal of Molecular Biology·B DahlmannP M Kloetzel
Nov 4, 2000·Nature Structural Biology·M GrollD Finley
Mar 16, 2001·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·D N. MiniatiR Robbins
Mar 27, 2001·Nature Reviews. Molecular Cell Biology·P M Kloetzel
Jun 8, 2001·Annual Review of Biochemistry·C M Pickart
Feb 1, 2002·Journal of Biochemistry·Masaki UnnoTomitake Tsukihara
Mar 1, 2002·Advances in Protein Chemistry·P ZwicklW Baumeister
Mar 28, 2002·Physiological Reviews·Michael H Glickman, Aaron Ciechanover
May 17, 2002·Structure·Masaki UnnoTomitake Tsukihara
Jun 25, 2002·Advances in Immunology·Kenneth L RockAlfred L Goldberg
Mar 22, 2003·Current Opinion in Cell Biology·Christopher L Brooks, Wei Gu
Apr 4, 2003·The International Journal of Biochemistry & Cell Biology·Cezary Wójcik, George N DeMartino
Apr 4, 2003·The International Journal of Biochemistry & Cell Biology·Kenneth J Rodgers, Roger T Dean
Apr 11, 2003·Reviews of Physiology, Biochemistry and Pharmacology·E KrügerP-M Kloetzel
Nov 13, 2003·Molecular and Cellular Biology·Mi-Kyoung KwakThomas W Kensler
Jun 30, 2004·Cellular and Molecular Life Sciences : CMLS·M Bajorek, M H Glickman
Jan 18, 2005·Trends in Cell Biology·Martin Rechsteiner, Christopher P Hill
Nov 9, 2005·Methods in Enzymology·Suzanne ElsasserDaniel Finley
Nov 9, 2005·Methods in Enzymology·Maria Gaczynska, Pawel A Osmulski
Dec 13, 2005·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Rochelle Buffenstein
Jan 21, 2006·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Yuji IkenoJames F Nelson
Oct 5, 2006·Molecular Cell·Chang-Wei LiuGeorge N DeMartino
Oct 24, 2006·Aging Cell·Blazej AndziakRochelle Buffenstein
Mar 7, 2007·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·João Pedro de MagalhãesGeorge M Church
Apr 7, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Vita A VernaceMaria E Figueiredo-Pereira
Apr 10, 2007·FEBS Letters·John Hanna, Daniel Finley
Jan 9, 2008·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Rochelle Buffenstein
Mar 19, 2008·Journal of Neurochemistry·Deborah A FerringtonDale S Gregerson
Jul 19, 2008·IUBMB Life·Tobias Jung, Tilman Grune
Jan 24, 2009·Journal of Molecular Biology·James M BaughEvgeny V Pilipenko

❮ Previous
Next ❯

Citations

Apr 2, 2014·Oxidative Medicine and Cellular Longevity·Daniel Ortuño-SahagúnArgelia E Rojas-Mayorquín
May 2, 2014·BioMed Research International·Izabela Sadowska-Bartosz, Grzegorz Bartosz
Feb 20, 2016·Cell Stress & Chaperones·Karl A RodriguezRochelle Buffenstein
Aug 2, 2015·Free Radical Biology & Medicine·T Keith BlackwellMeltem Isik
Sep 6, 2015·Proceedings of the National Academy of Sciences of the United States of America·Kexin LiHuabin Zhao
Sep 8, 2015·Experimental Gerontology·Samantha J AlperJames M Harper
Oct 31, 2012·Molecular Aspects of Medicine·Niki ChondrogianniEfstathios S Gonos
Jan 15, 2016·Aging Cell·Anna B SunshineMaitreya J Dunham
Sep 8, 2015·Experimental Gerontology·Jamila R SanchezRochelle Buffenstein
Apr 8, 2015·Mechanisms of Ageing and Development·Valerie VanhoorenBertrand Friguet
Jun 30, 2015·Scientific Reports·Orsolya K PenzErik Keimpema
Apr 10, 2016·Archives of Biochemistry and Biophysics·F Triana-MartínezM Königsberg
Sep 30, 2014·Mechanisms of Ageing and Development·Elena BellavistaClaudio Franceschi
Dec 30, 2014·Ageing Research Reviews·Niki ChondrogianniEfstathios S Gonos
Apr 7, 2015·Archives of Biochemistry and Biophysics·Valentina R GarbarinoRochelle Buffenstein
Apr 14, 2015·The Journal of Clinical Investigation·Andrew M PickeringRichard A Miller
Feb 18, 2015·Annual Review of Animal Biosciences·Sarah J MitchellRafael de Cabo
Nov 22, 2014·Frontiers in Molecular Neuroscience·Karl A RodriguezRochelle Buffenstein
Dec 19, 2013·International Journal of Cell Biology·Karina Cuanalo-ContrerasClaudio Soto
Oct 19, 2016·Immunology and Cell Biology·Gagandeep Kaur, Sanjay Batra
Jul 2, 2016·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Kaitlyn N LewisRochelle Buffenstein
Sep 17, 2016·Scientific Reports·Anna Maria SantoroDanilo Milardi
Jul 4, 2019·PLoS Computational Biology·Andrea KomljenovicMarc Robinson-Rechavi
Oct 23, 2019·The Journal of Experimental Biology·Vu Chau NguyenMatthew E Pamenter
Nov 19, 2019·Proteomics·Gregory TomblineVera Gorbunova
Jun 10, 2020·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Bharath SunchuViviana I Perez
Oct 18, 2019·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Yoshiyuki ArataShigeo Murata
Mar 17, 2015·Proceedings of the National Academy of Sciences of the United States of America·Kaitlyn N LewisRochelle Buffenstein
Feb 17, 2017·Physiological Reviews·Vladimir P SkulachevViktor A Sadovnichii
Mar 11, 2020·Proceedings of the National Academy of Sciences of the United States of America·Mikhail Y VyssokikhVladimir P Skulachev
Mar 19, 2019·Oxidative Medicine and Cellular Longevity·Frédéric SaldmannGérard Friedlander
Dec 25, 2019·Biochemistry. Biokhimii︠a︡·N A Popov, V P Skulachev
May 14, 2020·Nature Communications·Masaki TakasugiVera Gorbunova
Feb 26, 2014·Physiological Research·T A BakerM Majetschak
Mar 1, 2021·Redox Biology·Mary Α VasilopoulouNiki Chondrogianni
Jul 1, 2018·Free Radical Biology & Medicine·Laura C D Pomatto, Kelvin J A Davies
May 4, 2021·Frontiers in Cell and Developmental Biology·Shenae L CafeElizabeth G Bromfield

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
Protein Assay

Software Mentioned

Excel
ImageJ
SigmaPlot

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Related Papers

Mechanisms of Ageing and Development
Karl A RodriguezPawel A Osmulski
The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences
Kelly M GrimesRochelle Buffenstein
Antioxidants & Redox Signaling
Kaitlyn N LewisRochelle Buffenstein
Proceedings of the National Academy of Sciences of the United States of America
Jorge AzpuruaAndrei Seluanov
© 2021 Meta ULC. All rights reserved