Altered distribution of tight junction proteins after intestinal ischaemia/reperfusion injury in rats.

Journal of Cellular and Molecular Medicine
Qiurong LiJieshou Li

Abstract

Tight junction (TJ) disruptions have been demonstrated both in vitro and more recently in vivo in infection. However, the molecular basis for changes of TJ during ischaemia-reperfusion (I/R) injury is poorly understood. In the present study, intestinal damage was induced by I/R in an animal model. As assessed by TUNEL and propidium iodide uptake, we showed that I/R injury induced apoptosis as well as necrosis in rat colon, and the frequency of apoptotic and necrotic cells reached the maximum at 5 hrs of reperfusion. Immunofluorescence microscopy revealed that claudins 1, 3 and 5 are strongly expressed in the surface epithelial cells of the colon; however, labelling of all three proteins was present diffusely within cells and no longer focused at the lateral cell boundaries after I/R. Using Western blot analysis, we found that distribution of TJ proteins in membrane microdomains of TJ was markedly affected in I/R injury rats. Occludin, ZO-1, claudin-1 and claudin-3 were completely displaced from TX-100 insoluble fractions to TX-100 soluble fractions, and claudin-5 was partly displaced. The distribution of lipid raft marker protein caveolin-1 was also changed after I/R. I/R injury results in the disruption of TJs, which character...Continue Reading

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Citations

Jan 18, 2011·Antioxidants & Redox Signaling·Ingolf E BlasigReiner F Haseloff
Jul 14, 2012·American Journal of Physiology. Renal Physiology·Michael T EadonPatrick N Cunningham
Dec 21, 2013·Acta Neuropathologica·Gijs KooijHelga E de Vries
Sep 13, 2014·European Journal of Oral Sciences·Liang ShiFeng-Cai Wei
Mar 5, 2016·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Shuying TianHongyu Jiang
Oct 21, 2016·American Journal of Physiology. Cell Physiology·Younggeon Jin, Anthony T Blikslager
Feb 7, 2020·International Journal of Molecular Sciences·Zachary M Slifer, Anthony T Blikslager
May 15, 2020·The Journal of Toxicological Sciences·Ping GengBingyu Ling
Aug 3, 2019·Burns and Trauma·Wen HeFengjun Wang
Oct 16, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Xubiao WeiManyi Zhang
Dec 17, 2021·Experimental Gerontology·Kevin M NajarroElizabeth J Kovacs

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Methods Mentioned

BETA
electron microscopy
SMA
confocal microscopy
light microscopy
transmission electron microscopy

Software Mentioned

Quantity One
SigmaStat

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