Altered DNA methylation in liver and adipose tissues derived from individuals with obesity and type 2 diabetes

BMC Medical Genetics
Francisco Barajas-OlmosLorena Orozco

Abstract

Obesity is a well-recognized risk factor for insulin resistance and type 2 diabetes (T2D), although the precise mechanisms underlying the relationship remain unknown. In this study we identified alterations of DNA methylation influencing T2D pathogenesis, in subcutaneous and visceral adipose tissues, liver, and blood from individuals with obesity. The study included individuals with obesity, with and without T2D. From these patients, we obtained samples of liver tissue (n = 16), visceral and subcutaneous adipose tissues (n = 30), and peripheral blood (n = 38). We analyzed DNA methylation using Illumina Infinium Human Methylation arrays, and gene expression profiles using HumanHT-12 Expression BeadChip Arrays. Analysis of DNA methylation profiles revealed several loci with differential methylation between individuals with and without T2D, in all tissues. Aberrant DNA methylation was mainly found in the liver and visceral adipose tissue. Gene ontology analysis of genes with altered DNA methylation revealed enriched terms related to glucose metabolism, lipid metabolism, cell cycle regulation, and response to wounding. An inverse correlation between altered methylation and gene expression in the four tissues was found in a subset o...Continue Reading

References

Jun 28, 2005·Obesity Surgery·Ancha BaranovaZobair M Younossi
Dec 15, 2006·Nature·Steven E KahnKristina M Utzschneider
May 10, 2008·Bioinformatics·Pan DuSimon M Lin
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
Apr 20, 2010·Cancer Cell·Houtan NoushmehrUNKNOWN Cancer Genome Atlas Research Network
Mar 6, 2012·Cell·Varman T Samuel, Gerald I Shulman
May 23, 2012·Vascular Pharmacology·Cristiano Capurso, Antonio Capurso
Aug 17, 2012·Arteriosclerosis, Thrombosis, and Vascular Biology·Christian Rask-Madsen, C Ronald Kahn
Nov 13, 2012·Life Sciences·Manouchehr NakhjavaniAlireza Esteghamati
Nov 22, 2012·Population Health Metrics·Gretchen A StevensUNKNOWN Global Burden of Metabolic Risk Factors of Chronic Diseases Collaborating Group (Body Mass Index)
Jan 4, 2013·Diabetes Care·UNKNOWN American Diabetes Association
Dec 18, 2013·Bioinformatics·Tiffany J MorrisStephan Beck
Mar 14, 2014·The Lancet. Diabetes & Endocrinology·Norbert StefanMatthias B Schulze
Jan 22, 2015·Nucleic Acids Research·Matthew E RitchieGordon K Smyth
Feb 20, 2015·Nature·UNKNOWN Roadmap Epigenomics ConsortiumManolis Kellis
May 30, 2015·Trends in Pharmacological Sciences·Mathias Fasshauer, Matthias Blüher
Jun 5, 2015·Globalization and Health·Josiemer MatteiUNKNOWN Global Nutrition Epidemiologic Transition Initiative
Nov 7, 2015·Biochimie·Pauline MorignyDominique Langin
Jan 5, 2016·Cell Metabolism·Stephanie von Holstein-RathlouMatthew J Potthoff
Jan 20, 2016·Journal of Diabetes and Its Complications·Caroline Pereira DominguetiAna Paula Fernandes
Jun 28, 2016·Nature Reviews. Genetics·C David Allis, Thomas Jenuwein
Aug 2, 2016·Translational Research : the Journal of Laboratory and Clinical Medicine·A B CrujeirasJ M Fernandez-Real
Aug 2, 2016·Nature Reviews. Genetics·Heng ZhuJiang Qian
Aug 19, 2016·Diabetologia·Peter ArnerIngrid Dahlman
Feb 22, 2017·Translational Research : the Journal of Laboratory and Clinical Medicine·Frédéric GuénardMarie-Claude Vohl
Mar 3, 2017·Diabetology & Metabolic Syndrome·Priscila SalaDaniel Giannella-Neto
Mar 10, 2017·Epigenetics : Official Journal of the DNA Methylation Society·Vanessa D de MelloJussi Pihlajamäki
Sep 28, 2017·Clinical Epigenetics·Sonia García-CalzónCharlotte Ling

❮ Previous
Next ❯

Citations

May 30, 2019·International Journal of Molecular Sciences·Marcin WłodarczykMarek Fol
Dec 5, 2019·Diabetology & Metabolic Syndrome·Matthew D BarberioMonica J Hubal
Aug 2, 2019·Endocrine, Metabolic & Immune Disorders Drug Targets·Kaiser Un Nisa, Mohammad Irshad Reza
May 2, 2019·Frontiers in Endocrinology·Andrea G Izquierdo, Ana B Crujeiras
Oct 19, 2019·British Journal of Pharmacology·Megan BeetchBarbara Stefanska
Sep 11, 2019·Molecular Metabolism·Marie LohJohn Campbell Chambers
Nov 7, 2020·Investigative Ophthalmology & Visual Science·Hui ChenFeng Wen
May 29, 2021·Frontiers in Endocrinology·Hannah MaudeInês Cebola
Jun 30, 2021·Nature Communications·Min-A JhunThemistocles L Assimes
Jul 23, 2021·Frontiers in Endocrinology·Sara AndradeMariana P Monteiro
Oct 1, 2021·Endocrine Journal·Chunhu WangJiguang Ma

❮ Previous
Next ❯

Methods Mentioned

BETA
biopsies
Methylation assay
chip

Software Mentioned

Lumi
Illumina GenomeStudio
ChAMP
RefbaseEWAS
Bioconductor
Database for Annotation , Visualization , and Integrated Disco...
R
GenomeStudio

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.