PMID: 8594202Feb 23, 1996Paper

Altered domain closure and iron binding in transferrins: the crystal structure of the Asp60Ser mutant of the amino-terminal half-molecule of human lactoferrin

Journal of Molecular Biology
H R FaberE N Baker

Abstract

The crystal structure of a site-specific mutant of the N-terminal half-molecule of human lactoferrin, Lf(N), in which the iron ligand Asp60 has been mutated to Ser, has been determined at 2.05 A resolution in order to determine the effects of the mutation on iron binding and domain closure. Yellow monoclinic crystals of the D60S mutant, in its iron-bound form, were prepared, and have unit cell dimensions a = 110.2 A, b = 57.0 A, c = 55.2 A, beta = 97.6 degrees, space group C2, with one molecule of 333 residues in the asymmetric unit. The structure was determined by molecular replacement, using the wild-type Lf(N) as search model, and was refined by restrained least-squares methods. The final model, comprising 2451 protein atoms (from residues 2 to 315) one Fe3+ and one CO2-(3), and 107 water molecules, gives an R-factor of 0.175 for all data in the resolution range 20.0 to 2.05 A. The model conforms well with standard geometry, having root-mean-square deviations of 0.014 A and 1.2 degrees from standard bond lengths and angles. The structure of the D60S mutant deviates in two important respects from the parent Lf(N) molecule. At the mutation site the Ser side-chain neither binds to the iron atom nor makes any interdomain contact...Continue Reading

Citations

Apr 5, 2002·Medicinal Research Reviews·Hongyan Li, Zhong Ming Qian
Mar 19, 2003·Proceedings of the National Academy of Sciences of the United States of America·Heather M BakerEdward N Baker
Aug 31, 2010·The Biochemical Journal·Husain K KhambatiAnthony B Schryvers
Nov 13, 2010·Metallomics : Integrated Biometal Science·Joshua J LensbouerRobert P Doyle
Jan 22, 2013·Journal of Food Science·Suzanne M Jervis, MaryAnne Drake
Nov 13, 2007·Journal of Food Science·Gen-Hung ChenShann-Tzong Jiang
Aug 17, 2000·Bioscience, Biotechnology, and Biochemistry·M Hirose
Dec 26, 2001·Chemical Reviews·H SunP J Sadler
Feb 12, 1998·Journal of Molecular Biology·S A MooreE N Baker
Oct 3, 2002·Protein Expression and Purification·M ParamasivamA Srinivasan
Aug 23, 2005·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Lisa A LambertAnne B Mason

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