PMID: 6160583Oct 1, 1980Paper

Altered enolase in aged Turbatrix aceti results from conformational changes in the enzyme

Proceedings of the National Academy of Sciences of the United States of America
H K Sharma, M Rothstein

Abstract

Young- and old-type enolases (2-phospho-D-glycerate hydrolyase, EC 4.2.1.11) from the free-living nematode Turbatrix aceti can be unfolded in 1.25 M guanidine hydrochloride and subsequently refolded with essentially a quantitative recovery. After refolding, both enolases form an identical or near-identical third type of the enzyme as determined by spectral criteria, sensitivity to heat, immunotitration, and rate of inactivation by bacterial protease. By the same criteria, the refolded enolase is closer in conformation to the native old form of the enzyme than to the young form. The results prove that young and old enolases are conformational isomers and that an in vivo transformation from young to old enzyme takes place by conformational changes without covalent modification. The process may be related to the previously demonstrated slowing of enolase turnover in T. aceti. Errors in sequence cannot be involved in the age-related alteration of the enzyme.

References

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Citations

Oct 1, 1996·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·G J Lithgow
Mar 17, 2007·Molecular Genetics and Genomics : MGG·Koen Houthoofd, Jacques R Vanfleteren
Apr 1, 2000·Journal of the American Aging Association·B P BraeckmanJ R Vanfleteren
Jun 1, 1984·Mechanisms of Ageing and Development·H K Sharma, M Rothstein
Jan 1, 1984·Mechanisms of Ageing and Development·N Indrapal Singh, K Subba Rao
Nov 1, 1985·Mechanisms of Ageing and Development·M VelezJ Satrústegui
Sep 20, 1985·Biochimica Et Biophysica Acta·H Hardt, M Rothstein
Mar 1, 1991·Archives of Gerontology and Geriatrics·A MachadoC Santa Maria
Jun 15, 2004·Medical Hypotheses·C W Jameson
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·K C Yuh, A Gafni
Jul 11, 2001·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·G A WalkerG J Lithgow
Dec 15, 1988·European Journal of Biochemistry·R Takahashi, S Goto
Jul 26, 1982·Biochimica Et Biophysica Acta·L S Hiremath, M Rothstein
Feb 1, 1979·Mechanisms of Ageing and Development·M Rothstein
Jan 5, 2016·Structure·Adam M R de GraffKen A Dill
Mar 19, 2008·Trends in Cell Biology·Nektarios Tavernarakis
Sep 1, 1984·Archives of Biochemistry and Biophysics·J A Miernyk, D T Dennis
Oct 3, 2006·Experimental Gerontology·Thomas E Johnson
Oct 1, 1987·Journal of the American Geriatrics Society·C N OliverE R Stadtman
Feb 7, 1991·Journal of Theoretical Biology·R F RosenbergerE Kolettas
Aug 12, 2016·The Journal of Physical Chemistry. B·Kingshuk GhoshKen A Dill

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