Altered Epiphytic Colonization of Mannityl Opine-Producing Transgenic Tobacco Plants by a Mannityl Opine-Catabolizing Strain of Pseudomonas syringae.

Applied and Environmental Microbiology
M WilsonS E Lindow

Abstract

The plasmid pYDH208, which confers the ability to catabolize the mannityl opines mannopine and agropine, was mobilized into the nonpathogenic Pseudomonas syringae strain Cit7. The growth of the mannityl opine-catabolizing strain Cit7(pYDH208) was compared with that of the near-isogenic non-opine-catabolizing strain Cit7xylE on leaves of wild-type tobacco (Nicotiana tabacum cv. Xanthi) and transgenic mannityl opine-producing tobacco plants (N. tabacum cv. Xanthi, line 2-26). The population size of Cit7(pYDH208) was significantly greater on the lower leaves of transgenic plants than on middle or upper leaves of those plants. The population size of Cit7(pYDH208) on lower leaves of transgenic plants was also significantly greater than the population size of Cit7xylE on similar leaves of wild-type plants. High-voltage paper electrophoresis demonstrated higher levels of mannityl opines in washings from lower- and mid-level leaves than in washings from upper-level leaves. The ability of Cit7(pYDH208) to catabolize mannityl opines in the carbon-limited phyllosphere increased the carrying capacity of the lower leaves of transgenic plants for Cit7(pYDH208). In coinoculations, the increase in the ratio of population sizes of Cit7(pYDH208)...Continue Reading

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Citations

Feb 5, 1998·Molecular Plant-microbe Interactions : MPMI·H Kim, S K Farrand
Sep 19, 2002·Molecular Plant-microbe Interactions : MPMI·Michael A SavkaSilvia Rossbach
Sep 7, 2000·Microbiology and Molecular Biology Reviews : MMBR·S S Hirano, C D Upper
Mar 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·J H Leveau, S E Lindow
Jan 5, 2000·Applied and Environmental Microbiology·J Mercier, S E Lindow
Sep 10, 2016·The Journal of Biological Chemistry·Loïc MartySolange Moréra
Dec 3, 1998·Applied and Environmental Microbiology·B B M Gardener, de Bruijn FJ
Jan 5, 2002·Applied and Environmental Microbiology·Bhinu V S Pillai, Sanjay Swarup
Jan 20, 2012·The ISME Journal·Mitja N P Remus-EmsermannJohan H J Leveau
Jun 12, 2013·Environmental Microbiology Reports·Mitja N P Remus-EmsermannJohan H J Leveau
Sep 1, 1996·Applied and Environmental Microbiology·L L KinkelS E Lindow
Jul 1, 2021·Phytochemistry·Tatiana Matveeva, Léon Otten

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