Altered erythrocyte membrane characteristics during anemia in childhood acute lymphoblastic leukemia

Annals of Hematology
Shyamasree GhoshChitra Mandal

Abstract

Anemia is a prominent feature in children with acute lymphoblastic leukemia (ALL). To investigate the erythrocyte features during anemia in these patients, we studied the altered characters of these cells and oxidative stress imposed in their serum. This investigation reveals that erythrocytes from ALL patients show (1) increased membrane fluidity detected by fluorescence anisotropy studies, increased osmotic fragility detected by hemolysis of erythrocytes in different saline concentrations, and increased hydrophobicity as measured by binding with 8-anilino-1-naphthalenesulfonic acid, (2) enhanced (approximately threefold) glycosylation and sialylation, monitored by digoxigenin enzyme assay, and (3) expression of disease-specific 210, 105, 83, 54, and 28 kDa 9-O-acetyl sialoglycoconjugates (9-O-AcSGs) demonstrated by Western blot analysis and fluorescence-activated cell sorter (FACS) analysis studies using Achatinin-H with specificity towards 9-O-AcSAalpha2-6GalNAc as the analytical probe. (4) In addition, induced oxidative stress was observed in the sera of these children as indicated by increased nitric oxide (approximately fourfold) and thiobarbituric acid (TBA) reactive species (twofold) as detected by Griess reaction and T...Continue Reading

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Citations

May 13, 2006·Glycoconjugate Journal·Waliza AnsarChitra Mandal
Mar 20, 2009·Biotechnology Journal·Suchandra Chowdhury, Chitra Mandal
Mar 3, 2007·The Journal of Pharmacy and Pharmacology·Mohammed M H Al-GayyarAmal M El-Gayar
Aug 1, 2006·Pathophysiology : the Official Journal of the International Society for Pathophysiology·Durjoy MajumderAbhijit Chakrabarti
Mar 18, 2015·Glycoconjugate Journal·Shyamasree Ghosh
Aug 22, 2017·Indian Journal of Hematology & Blood Transfusion : an Official Journal of Indian Society of Hematology and Blood Transfusion·Fatime GeyikoğluMerve Sönmez
Jul 31, 2018·Glycoconjugate Journal·Shyamasree Ghosh
Jun 9, 2021·International journal for vitamin and nutrition research. Internationale Zeitschrift für Vitamin- und Ernährungsforschung. Journal international de vitaminologie et de nutrition·Isa KiranÖzge Vural

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