Altered expression levels of occludin, claudin-1 and myosin light chain kinase in the common bile duct of pediatric patients with pancreaticobiliary maljunction

BMC Gastroenterology
Shun-Gen HuangJian Wang

Abstract

In pancreaticobiliary maljunction (PBM), the sphincter of Oddi can not control bile and pancreatic juice flow, which may lead to two-way reflux of bile and pancreatic juice, thus causing chronic inflammation, thickening, fibrosis and metaplasia of the common bile duct wall. These pathophysiological changes have been linked to disruption of the epithelium barrier in the common bile duct. We hypothesized that the expression of tight junction-associated proteins may be dysregulated in the common bile duct in PBM. In the current study, we sought to analyze the expression of tight junction-associated proteins in the common bile duct epithelium of pediatric patients with PBM. Specimens of the common bile duct were collected from 12 pediatric patients with PBM and 10 non-PBM controls. The expression of the tight junction-associated proteins occludin and claudin-1 in the epithelium was examined by immunohistochemistry. The Image-Pro Plus v. 6.0 image analysis software was used to calculate the mean qualifying score (MQS) of imunostained sections of common bile duct epithelium. Total protein extracts of common bile duct were analyzed by Western blotting assays to examine expression of occludin, claudin-1 and myosin light chain kinase (M...Continue Reading

References

Jan 1, 1982·The American Journal of Physiology·P J Reiser, B T Stokes
Oct 1, 1995·The American Journal of Physiology·M B FallonJ M Anderson
Oct 1, 1994·Mayo Clinic Proceedings·R H Wiesner
Jun 1, 1993·The American Journal of Physiology·M B FallonJ M Anderson
Apr 29, 1998·Annual Review of Physiology·L L Mitic, J M Anderson
Sep 1, 2000·Seminars in Cell & Developmental Biology·J R Turner
Jun 16, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·N GasslerF Autschbach
Mar 19, 2003·Liver International : Official Journal of the International Association for the Study of the Liver·Shinichiro HanadaMichio Sata
Feb 8, 2006·Annual Review of Physiology·Christina M Van Itallie, James M Anderson
Apr 21, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·P ShethR K Rao
Dec 5, 2009·Clinical Gastroenterology and Hepatology : the Official Clinical Practice Journal of the American Gastroenterological Association·Terumi KamisawaTsuneo Sasaki
Jan 13, 2010·Cold Spring Harbor Perspectives in Biology·James M Anderson, Christina M Van Itallie
Jan 19, 2010·Annual Review of Pathology·Amanda M MarchiandoJerrold R Turner
May 25, 2011·Laboratory Investigation; a Journal of Technical Methods and Pathology·Srikar R GuntakaRadhakrishna Rao
May 16, 2012·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·M CamilleriG N Verne
Jun 27, 2012·Annals of the New York Academy of Sciences·Kevin E Cunningham, Jerrold R Turner
Mar 26, 2014·Tissue Barriers·R K Rao, G Samak

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Citations

Nov 21, 2019·Nature Communications·M Ilcim ThestrupElke A Ober
Jul 3, 2018·Gastroenterology·Tirthadipa Pradhan-SunddPrithu Sundd
Jan 17, 2019·Gene Expression·Tirthadipa Pradhan-Sundd, Satdarshan Pal Monga

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