Altered expression, localization, and phosphorylation of epithelial junctional proteins in celiac disease

American Journal of Clinical Pathology
Rachele CiccocioppoG R Corazza

Abstract

We aimed to study the expression and localization of the molecular components of enterocyte junctions in celiac disease together with the level of tyrosine phosphorylation, a phenomenon known to affect their cellular distribution and function, and to explore the influence of proinflammatory cytokines. Duodenal biopsy specimens from patients with celiac disease and control subjects were used for immunoprecipitation, immunoblotting, and immunolocalization by using antioccludin, anti-zonula occludens (ZO)-1, anti-E-cadherin, anti-beta-catenin, and antiphosphotyrosine antibodies. The same procedures were carried out on filter-grown Caco-2 cells incubated in the absence or presence of interferon g and tumor necrosis factor a. In active celiac disease, the absence of a phosphorylated ZO-1 and the extensive phosphorylation of beta-catenin might be responsible for the absence of membranous localization of occludin and E-cadherin, respectively. The in vitro system showed an influence of the cytokines on the assembly of these complexes that proved the opposite to celiac samples as far as tight junctions were concerned because the presence of a phosphorylated ZO-1 enables occludin to localize in the membrane.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Feb 14, 1998·Journal of Cellular Physiology·M J EngleD H Alpers
Aug 26, 1998·The American Journal of Pathology·J A Efstathiou, M Pignatelli
Nov 24, 1999·Biochimica Et Biophysica Acta·S GómezA García de Herreros
Dec 14, 1999·The Journal of Biological Chemistry·S RouraM Duñach
Aug 10, 2000·The British Journal of Surgery·B P WijnhovenM Pignatelli
Apr 11, 2001·American Journal of Clinical Pathology·R CiccocioppoG R Corazza
Mar 22, 2002·Journal of Clinical Gastroenterology·Mark T DeMeoMary C Tobin
Mar 4, 2003·Nature Reviews. Molecular Cell Biology·Karl Matter, Maria S Balda
Jan 21, 2004·The Journal of Surgical Research·Lisa S PoritzWalter A Koltun
Jan 22, 2004·The Biochemical Journal·Thilo HagenAntonio Vidal-Puig
Apr 1, 2004·Journal of Cell Science·Christian BojarskiOtmar Huber
May 21, 2004·American Journal of Physiology. Cell Physiology·Eveline E Schneeberger, Robert D Lynch
Jun 12, 2004·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·D PizzutiD Martines
Sep 15, 2004·Current Opinion in Cell Biology·Mariann Bienz, Fumihiko Hamada
Feb 16, 2005·Annals of Internal Medicine·Armin Alaedini, Peter H R Green
Mar 19, 2005·The EMBO Journal·Judith A TunggalCarien M Niessen
Apr 15, 2005·Molecular Immunology·Marco LondeiLuigi Maiuri

❮ Previous
Next ❯

Citations

Apr 22, 2008·PLoS Pathogens·Michael T Bethune, Chaitan Khosla
Nov 7, 2008·Journal of Clinical Immunology·Sara AshornSari Iltanen
Jan 8, 2009·Journal of Proteome Research·Jeffrey M SundstromDavid A Antonetti
Mar 19, 2011·American Journal of Physiology. Gastrointestinal and Liver Physiology·Rana Al-SadiThomas Ma
Jul 19, 2011·Gastroenterology·Dongmei YeThomas Y Ma
Apr 28, 2009·Lancet·Antonio Di Sabatino, Gino Roberto Corazza
Jul 28, 2009·Scandinavian Journal of Immunology·G DenizM Zouali
Jul 23, 2016·Canadian Journal of Gastroenterology & Hepatology·María Inés Pinto-SánchezJulio C Bai
Aug 14, 2013·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·P D MooneyD S Sanders

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.