Altered expression of miRNAs and mRNAs reveals the potential regulatory role of miRNAs in the developmental process of early weaned goats

PloS One
Rongrong LiaoYuexia Lin

Abstract

MicroRNAs (miRNAs) play pivotal roles in growth, development, and stress responses. However, the regulatory function of miRNAs in early weaned goats remains unclear. Deep sequencing comparison of mRNA and miRNA expression profiles showed that 18 miRNAs and 373 genes were differentially expressed in pre- and post-weaning Chongming white goats. Bioinformatics analysis indicated that these differentially expressed genes are involved in cellular processes, developmental processes, and growth in terms of biological process analysis. KEGG analysis showed that downregulated genes were enriched in salivary secretion, bile secretion, vascular smooth muscle contraction, and calcium signaling pathways. Additionally, a miRNA-mRNA co-expression network of the 18 dysregulated miRNAs and their 107 target mRNAs was constructed using a combination of Pearson's correlation analysis and prediction by miRanda software. Among the downregulated miRNAs, two (chi-miR-206 and chi-miR-133a/b) were muscle development-related and the others were cell proliferation associated. Further RT-qPCR analysis confirmed that downregulated miRNAs (chi-miR-99b-3p, chi-miR-224, and chi-miR-10b-5p) were highly expressed in muscle tissues (heart, spleen, or kidney) of t...Continue Reading

References

Mar 1, 2006·BMC Bioinformatics·Joshua S YuanC Neal Stewart
Sep 17, 2009·Journal of Cellular and Molecular Medicine·Tomoyuki NakasaMitsuo Ochi
May 18, 2010·Molecular Cell·Haruhiko Siomi, Mikiko C Siomi
Sep 14, 2010·Journal of Biochemistry·Brandi N Davis-Dusenbery, Akiko Hata
May 24, 2011·Nature Reviews. Molecular Cell Biology·Thomas Braun, Mathias Gautel
Dec 14, 2011·Cancer Prevention Research·Colin C PritchardMuneesh Tewari
Jan 21, 2012·Trends in Cell Biology·Xi ChenChen-Yu Zhang
Feb 24, 2012·Nature·Tom H CheungThomas A Rando
Oct 1, 2007·Animal : an International Journal of Animal Bioscience·P L Greenwood, L M Cafe
Apr 11, 2013·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Evangelos MavrommatisLeonidas C Platanias
Sep 10, 2014·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Mariko EsumiTakaji Wakita
Jan 18, 2015·The Journal of Biological Chemistry·Ali Hashemi GheinaniKatia Monastyrskaya
Jan 24, 2015·Current Vascular Pharmacology·Xin YuWilliam Ka Kei Wu
Feb 14, 2015·International Journal of Biological Sciences·Guoda MaKeshen Li
Nov 2, 2016·In Vitro Cellular & Developmental Biology. Animal·Wei Ran ZhangHong Guo
Nov 5, 2016·Frontiers in Bioscience (Landmark Edition)·Clayton YatesLara Sucheston-Campbell
May 10, 2017·Journal of Thermal Biology·Li ZhenHuanmin Yang
Aug 15, 2017·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Min HeJian Wang
Sep 20, 2017·International Journal of Medical Sciences·Jinzhe ZhouBujun Ge
Sep 28, 2017·Cancer Biomarkers : Section a of Disease Markers·Qin ShiMinshan Fang
Dec 5, 2017·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Ke WeiYijiang Chen
Aug 29, 2018·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Caihong WeiLixin Du
Sep 9, 2018·Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology·Yuxia YueYan Hong
Oct 30, 2018·Journal of Cellular Physiology·Che WangChuanyu Gao

❮ Previous
Next ❯

Citations

Mar 4, 2020·Animals : an Open Access Journal From MDPI·Yaseen UllahShengwei Hu
Jan 5, 2021·Frontiers in Veterinary Science·Silvia MirettiMario Baratta
Jan 10, 2021·Trends in Microbiology·Nilusha Malmuthuge, Le Luo Guan

❮ Previous
Next ❯

Datasets Mentioned

BETA
PRJNA507343
PRJNA507087

Methods Mentioned

BETA
PCR
Illumina sequencing

Software Mentioned

DESeq2
Cytoscape
miRanda

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.