Altered Expression of Wnt Signaling Pathway Components in Osteogenesis of Mesenchymal Stem Cells in Osteoarthritis Patients

PloS One
Pilar Tornero-EstebanJosé Ramón Lamas

Abstract

Osteoarthritis (OA) is characterized by altered homeostasis of joint cartilage and bone, whose functional properties rely on chondrocytes and osteoblasts, belonging to mesenchymal stem cells (MSCs). WNT signaling acts as a hub integrating and crosstalking with other signaling pathways leading to the regulation of MSC functions. The aim of this study was to evaluate the existence of a differential signaling between Healthy and OA-MSCs during osteogenesis. MSCs of seven OA patients and six healthy controls were isolated, characterised and expanded. During in vitro osteogenesis, cells were recovered at days 1, 10 and 21. RNA and protein content was obtained. Expression of WNT pathway genes was evaluated using RT-qPCR. Functional studies were also performed to study the MSC osteogenic commitment and functional and post-traslational status of β-catenin and several receptor tyrosine kinases. Several genes were downregulated in OA-MSCs during osteogenesis in vitro. These included soluble Wnts, inhibitors, receptors, co-receptors, several kinases and transcription factors. Basal levels of β-catenin were higher in OA-MSCs, but calcium deposition and expression of osteogenic genes was similar between Healthy and OA-MSCs. Interestingly an...Continue Reading

References

Mar 31, 1999·Proceedings of the National Academy of Sciences of the United States of America·J C HsiehJ Nathans
May 17, 2002·The New England Journal of Medicine·Lynn M BoydenRichard P Lifton
Feb 6, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Chaoxiang ShuiSundeep Khosla
Sep 10, 2003·Current Opinion in Rheumatology·Daniel Lajeunesse, Pascal Reboul
Jan 2, 2004·Aging Clinical and Experimental Research·Jan DequekerFrank P Luyten
Jan 30, 2004·The Journal of Biological Chemistry·Tamar GolanArnona Gazit
Oct 12, 2004·Gene·Jennifer J WestendorfTania M Schroeder
May 5, 2006·The Journal of Biological Chemistry·Yuan Gao, Hsien-yu Wang
Feb 14, 2007·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Wendy BalemansWim Van Hul
Oct 5, 2007·Blood·Ulrika BlankStefan Karlsson
Dec 7, 2007·Hormones : International Journal of Endocrinology and Metabolism·Maria P Yavropoulou, John G Yovos
Mar 11, 2008·Cell·Toshio Suda, Fumio Arai
May 6, 2008·Best Practice & Research. Clinical Rheumatology·Rik J U Lories
Sep 30, 2008·Nature Clinical Practice. Rheumatology·Maripat Corr
Nov 20, 2008·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Hiroshi Kawaguchi
Jan 13, 2009·Gene·Ling LingSimon M Cool
May 15, 2009·Arthritis Research & Therapy·Rik J U LoriesKurt de Vlam
Nov 18, 2009·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Anthony D PersonJamie L Lohr
Feb 5, 2010·Best Practice & Research. Clinical Rheumatology·Steeve Kwan TatJohanne Martel-Pelletier
Nov 12, 2010·International Journal of Molecular Medicine·Yuan YuanWei Ke Si
Dec 8, 2010·Nature Reviews. Rheumatology·Rik J Lories, Frank P Luyten
Feb 3, 2011·The Journal of Biological Chemistry·Ondrej BernatikVitezslav Bryja
Feb 26, 2011·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Jin ZhangJake Chen
Sep 10, 2011·Acta Biochimica Et Biophysica Sinica·Antara De
Dec 20, 2011·Bone·Kay Chapman, Ana M Valdes
Jan 24, 2012·Best Practice & Research. Clinical Rheumatology·Farshid Guilak
Jan 26, 2012·Frontiers in Molecular Neuroscience·Caroline N MillsEddy S Yang
Jul 11, 2012·Stem Cell Research & Therapy·Pawan K GuptaAnish S Majumdar
Jan 9, 2013·Expert Opinion on Therapeutic Targets·Robert J MacFarlaneEleftherios Tsiridis
Feb 8, 2013·Nature Medicine·Roland Baron, Michaela Kneissel
Mar 6, 2013·Nature Reviews. Rheumatology·Rik J LoriesNancy E Lane
May 7, 2013·Current Molecular Medicine·J Stagg, J Galipeau
Jun 4, 2013·Annual Review of Cell and Developmental Biology·Matthew B GreenblattLaurie H Glimcher

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Citations

Oct 18, 2016·Frontiers in Physiology·Bipradas RoyHugh M Fentress
Apr 21, 2017·Journal of Cellular Physiology·Nicola MaruottiFrancesco P Cantatore
Dec 19, 2016·European Journal of Cell Biology·Sajjad SisakhtnezhadHassan Akrami
Aug 18, 2016·In Vitro Cellular & Developmental Biology. Animal·Guijuan FengDongmei Zhang
Jun 9, 2018·Biomedicines·Jessika BertacchiniCarla Palumbo
Nov 11, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Armita Mahdavi GorabiAmirhossein Sahebkar
Sep 25, 2019·International Journal of Molecular Sciences·Béla KovácsElőd Ernő Nagy
Feb 24, 2021·Connective Tissue Research·Laura López-DelgadoJose A Riancho
Mar 7, 2021·Cells·Arkaitz MucientesBenjamín Fernández-Gutiérrez
Jul 17, 2021·EFORT Open Reviews·Gerardo FuscoElizaveta Kon

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Methods Mentioned

BETA
phase contrast microscopy
Profiler
PCR
Antibody Array
Enzyme-Linked Immunosorbent Assay
Protein assay
Flow Citometry
flow Cytometry
ELISA

Software Mentioned

ImageJ

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