PMID: 9180926May 1, 1997Paper

Altered gene expression in spontaneous hepatocellular carcinomas from male B6C3F1 mice

Molecular Carcinogenesis
E H RomachT R Fox

Abstract

In this study, we analyzed spontaneous hepatocellular carcinomas (HCCs) from male B6C3F1 mice for alterations in the expression of the genes for c-myc, insulin-like growth factor II (IGF-II), cyclin D1, transforming growth factor-alpha (TGF-alpha), and the epidermal growth factor receptor (EGFR). These genes are all important in growth control in the rodent liver, and therefore, alterations in these genes or their products may result in unregulated growth. Northern blot analysis demonstrated an increase in expression of c-myc mRNA in five of 21 (24%) spontaneous HCCs compared with nontumor tissue. Tumors that had an increase in c-myc mRNA did not have an amplified c-myc gene. Of the HCCs analyzed, 18 of 29 (62%) showed reexpression of IGF-II RNA when compared with controls. Cyclin D1 mRNA was overexpressed in seven of 27 (26%) of the tumors analyzed relative to controls. Tumors with an increase in cyclin D1 mRNA also overexpressed the cyclin D1 protein. RNA encoding for the EGFR was decreased in 21 of 23 (91%) HCCs when compared with controls. None of the 29 liver tumors analyzed for alterations in expression of TGF-alpha mRNA differed from controls. Also, each individual tumor had a unique set of molecular alterations even whe...Continue Reading

References

Apr 11, 1991·Nature·T MotokuraA Arnold
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J E Mead, N Fausto
Mar 13, 1986·Biochemical and Biophysical Research Communications·D L HwangB I Carr
Jan 1, 1987·Archives of Toxicology. Supplement. = Archiv Für Toxikologie. Supplement·R R MaronpotJ E Huff
May 1, 1981·The Journal of Clinical Investigation·H S Earp, E J O'Keefe
Aug 30, 1984·Biochemical and Biophysical Research Communications·J BlattT P Foley
Apr 1, 1995·Carcinogenesis·A I Robles, C J Conti
Oct 29, 1993·Biochemical and Biophysical Research Communications·Y J ZhangI B Weinstein
Jan 1, 1993·Annual Review of Physiology·W S Cohick, D R Clemmons
Mar 1, 1993·Molecular and Cellular Endocrinology·P D Gluckman, G R Ambler

❮ Previous
Next ❯

Citations

Aug 8, 2001·International Journal of Experimental Pathology·J R Foster
Jul 20, 2002·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Harald LahmEckhard Wolf

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.