Altered Glucosinolate Profiles and Expression of Glucosinolate Biosynthesis Genes in Ringspot-Resistant and Susceptible Cabbage Lines

International Journal of Molecular Sciences
Md AbuyusufIll-Sup Nou

Abstract

Ringspot, caused by the fungus Mycosphaerella brassicicola, is a serious disease of Brassica crops worldwide. Despite noteworthy progress to reveal the role of glucosinolates in pathogen defense, the host⁻pathogen interaction between cabbage (Brassica oleracea) and M. brassicicola has not been fully explored. Here, we investigated the glucosinolate profiles and expression of glucosinolate biosynthesis genes in the ringspot-resistant (R) and susceptible (S) lines of cabbage after infection with M. brassicicola. The concomitant rise of aliphatic glucoiberverin (GIV) and indolic glucobrassicin (GBS) and methoxyglucobrassicin (MGBS) was linked with ringspot resistance in cabbage. Pearson's correlation and principle component analysis showed a significant positive association between GIV contents and the expression of the glucosinolate biosynthesis gene ST5b-Bol026202 and between GBS contents and the expression of the glucosinolate biosynthesis gene MYB34-Bol017062. Our results confirmed that M. brassicicola infection induces the expression of glucosinolate biosynthesis genes in cabbage, which alters the content of individual glucosinolates. This link between the expression of glucosinolate biosynthesis genes and the accumulation of...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jun 7, 2002·Trends in Plant Science·Ute Wittstock, Barbara A Halkier
Jan 17, 2003·Plant Physiology·Michael Dalgaard MikkelsenBarbara Ann Halkier
Aug 16, 2003·Journal of Chemical Ecology·Anurag A Agrawal, Nile S Kurashige
Apr 28, 2006·The Plant Journal : for Cell and Molecular Biology·Carina Barth, Georg Jander
May 20, 2006·The Plant Journal : for Cell and Molecular Biology·Günter BraderE Tapio Palva
Jun 7, 2006·Proceedings of the National Academy of Sciences of the United States of America·Markus BenderothJuergen Kroymann
Sep 25, 2008·Annual Review of Entomology·Richard J HopkinsJoop J A van Loon
May 23, 2013·Proceedings of the National Academy of Sciences of the United States of America·Kei HirumaYoshitaka Takano
Jan 17, 2014·Molecular Plant·Henning Frerigmann, Tamara Gigolashvili
May 24, 2014·Nature Communications·Shengyi LiuAndrew H Paterson
Jul 25, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Go-Eun YiIll-Sup Nou
Jun 21, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Arif Hasan Khan RobinIll-Sup Nou
Oct 27, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Go-Eun YiIll-Sup Nou
Aug 1, 1994·The New Phytologist·Richard N Bennett, Roger M Wallsgrove

❮ Previous
Next ❯

Methods Mentioned

BETA
NGBS
HGBS
PCA
reverse transcription quantitative PCR
PCR

Software Mentioned

Minitab
Excel
Light Cycler

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.