Altered glycan accessibility on native immunoglobulin G complexes in early rheumatoid arthritis and its changes during therapy

Clinical and Experimental Immunology
J StümerL E Muñoz

Abstract

The goal of this study was to investigate the glycosylation profile of native immunoglobulin (Ig)G present in serum immune complexes in patients with rheumatoid arthritis (RA). To accomplish this, lectin binding assays, detecting the accessibility of glycans present on IgG-containing immune complexes by biotinylated lectins, were employed. Lectins capturing fucosyl residues (AAL), fucosylated tri-mannose N-glycan core sites (LCA), terminal sialic acid residues (SNA) and O-glycosidically linked galactose/N-acetylgalactosamine (GalNac-L) were used. Patients with recent-onset RA at baseline and after 3-year follow-up were investigated. We found that native IgG was complexed significantly more often with IgM, C1q, C3c and C-reactive protein (CRP) in RA patients, suggesting alterations of the native structure of IgG. The total accessibility of fucose residues on captured immune complexes to the respective lectin was significantly higher in patients with RA. Moreover, fucose accessibility on IgG-containing immune complexes correlated positively with the levels of antibodies to cyclic citrullinated peptides (anti-CCP). We also observed a significantly higher accessibility to sialic acid residues and galactose/GalNAc glyco-epitopes in ...Continue Reading

References

May 1, 1973·Annals of the Rheumatic Diseases·P M Carter
Jan 19, 2000·Clinical Biochemistry·I GornikG Lauc
Jul 18, 2003·Clinica Chimica Acta; International Journal of Clinical Chemistry·Yuji ImafukuYuji Yamada
Aug 31, 2004·Arthritis and Rheumatism·Markus M J NielenBen A C Dijkmans
Nov 23, 2005·Circulation·Yehuda ShoenfeldYaniv Sherer
Aug 5, 2006·Science·Yoshikatsu KanekoJeffrey V Ravetch
Jun 7, 2007·Heart·C J EdwardsUNKNOWN Hertfordshire Cohort Study Group
Aug 25, 2009·Annals of the Rheumatic Diseases·James I RobinsonAnn W Morgan
Oct 10, 2009·Rheumatology International·Ewa Maria KratzIwona Katnik-Prastowska
Jul 1, 1997·Biochemistry and Molecular Biology International·S Kabir, G J Gerwig
Nov 18, 2009·Circulation·Tanya N MayadasNaotake Tsuboi
Feb 24, 2010·Arthritis and Rheumatism·Hans U SchererRené E M Toes
Jan 1, 2009·Proteomics. Clinical Applications·H Ulrich SchererManfred Wuhrer
Apr 17, 2012·The Journal of Allergy and Clinical Immunology·Carolin M OefnerMarc Ehlers
Jul 3, 2013·Experimental Dermatology·Mattias Collin, Marc Ehlers
Feb 21, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Joris van der HeijdenTaco W Kuijpers
Mar 12, 2015·Molecular & Cellular Proteomics : MCP·Rosina PlompManfred Wuhrer
Apr 19, 2015·Arthritis Research & Therapy·Kyoung-Woon KimHae-Rim Kim
Jul 18, 2015·Journal of Immunology Research·Christoph FickentscherMartin Herrmann
Aug 9, 2015·Proceedings of the National Academy of Sciences of the United States of America·Chin-Wei LinChi-Huey Wong

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Citations

Apr 27, 2021·Frontiers in Pharmacology·Riccardo CapecchiLorenzo Bertani

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