Altered growth of transgenic tobacco lacking leaf cytosolic pyruvate kinase

Plant Physiology
V L KnowlesW C Plaxton

Abstract

Previously, we reported that transformation of tobacco (Nicotiana tabacum L.) with a vector containing a potato cytosolic pyruvate kinase (PKc) cDNA generated two plant lines specifically lacking leaf PKc (PKc-) as a result of co-suppression. PKc deficiency in these primary transformants did not appear to alter plant development, although root growth was not examined. Here we report a striking reduction in root growth of homozygous progeny of both PKc- lines throughout development under moderate (600 microE m-2 s-1) or low (100 microE m-2 s-1) light intensities. When both PKc- lines were cultivated under low light, shoot and flower development were also delayed and leaf indentations were apparent. Leaf PK activity in the transformants was significantly decreased at all time points examined, whereas root activities were unaffected. Polypeptides corresponding to PKc were undetectable on immunoblots of PKc- leaf extracts, except in 6-week-old low-light-grown PKc- plants, in which leaf PKc expression appeared to be greatly reduced. The metabolic implications of the kinetic characteristics of partially purified PKc from wild-type tobacco leaves are discussed. Overall, the results suggest that leaf PKc deficiency leads to a perturbat...Continue Reading

References

Oct 15, 1991·The Biochemical Journal·F E Podestá, W C Plaxton
Sep 1, 1996·Archives of Biochemistry and Biophysics·Z Hu, W C Plaxton
Jun 1, 1996·Annual Review of Plant Physiology and Plant Molecular Biology·P. Meyer, H. Saedler
Jun 1, 1996·Annual Review of Plant Physiology and Plant Molecular Biology·William C. Plaxton
Jun 1, 1996·Annual Review of Plant Physiology and Plant Molecular Biology·Raymond CholletMarion H. O'Leary
Oct 1, 1992·Plant Physiology·S G Gottlob-McHughD T Dennis

❮ Previous
Next ❯

Citations

Nov 21, 2007·Mitochondrion·Ko Noguchi, Keisuke Yoshida
Mar 6, 2008·The Plant Journal : for Cell and Molecular Biology·Till K PellnyChristine H Foyer
Jan 13, 2015·Protein Expression and Purification·Evgenia L AuslenderJean Rivoal
Sep 24, 2013·Plant Physiology and Biochemistry : PPB·Natalia Muñoz-FambuenaM Carmen González-Mas
Oct 19, 2017·Plant, Cell & Environment·Franklin Magnum de Oliveira SilvaAdriano Nunes-Nesi
Nov 30, 2019·Plant Science : an International Journal of Experimental Plant Biology·Changfei GuanYong Yang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.