Altered immunochemical reactivity of Saccharomyces cerevisiae a-cells after alpha-factor-induced morphogenesis.

Journal of Bacteriology
P N Lipke, C E Ballou

Abstract

Antibodies were raised against Saccharomyces cerevisiae a-cells that had been exposed to the sex pheromone, alpha-factor. After adsorption of the antiserum with diploid cells, antibodies remained that reacted specifically with the mannan from haploid cells. The characteristic determinant was observed in mannan from pheromone-treated a-cells, in mannan from untreated alpha-cells, and at a much lower concentration, in mannan from control a-cells. The antigens from these three mannans appeared to be identical. The determinant was destroyed by mild-acid hydrolysis or periodate oxidation, but not by proteolysis or digestion with exo-alpha-mannanase. Mutants with altered mannan were unable to express the antigen. Complete acid hydrolysates mannan from alpha-factor-treated a-cells contained mannose, glucose, and N-acetylglucosamine. Partial acid hydrolysis, under conditions that destroyed the antigenic determinant, released only mannose and mannobiose. The mannose fraction was labeled to high specific activity during response of a-cells to alpha-factor if radioactive glucose was the carbon source. Neither alpha- not beta-D-mannopyranosyl phosphate was a hapten. The results are consistent with the presence of a haploid-specific antigen...Continue Reading

References

Jun 1, 1978·Journal of Bacteriology·G FehrenbacherJ Thorner
Feb 1, 1979·The Journal of Cell Biology·J S Tkacz, V L MacKay
Jul 1, 1976·Journal of Bacteriology·P N LipkeC E Ballou
Jan 1, 1976·Journal of Immunological Methods·J W Goding
Oct 26, 1971·Biochemistry·W C Raschke, C E Ballou
Jul 1, 1974·Analytical Biochemistry·S C MarchP Cuatrecasas
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May 22, 1970·Biochemical and Biophysical Research Communications·T R Thieme, C E Ballou
Jan 1, 1949·Acta Pathologica Et Microbiologica Scandinavica·O OUCHTERLONY

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Citations

Oct 6, 1983·Nature·J R Strazdis, V L MacKay
Nov 14, 1983·FEBS Letters·T RuizL Rodriguez
Dec 1, 1981·Journal of Bacteriology·K Terrance, P N Lipke
Sep 1, 1995·Microbiological Reviews·V J CidM Sánchez

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