PMID: 9433480Jan 20, 1998Paper

Altered in vitro spreading and cytoskeletal organization in human glioma cells by downregulation of urokinase receptor

Molecular Carcinogenesis
S K ChintalaJ S Rao

Abstract

The interaction of urokinase-type plasminogen activator (uPA) with its cell-surface receptor (uPAR) is implicated in diverse biological processes such as cell migration, tissue remodeling, and tumor cell invasion. Recent studies indicated that uPAR can act as an extracellular matrix receptor during cell adhesion. Recently, we showed that transfection of the human glioma cell line SNB19 with antisense uPAR resulted in downregulation of uPAR at both the mRNA and protein levels. In this study, we used SNB19 to determine how the presence or absence of uPAR promotes cell spreading and associated changes in cell morphology. Microscopic analysis of cell spreading revealed that antisense uPAR-transfected cells were larger, remained round, and did not spread efficiently over extracellular matrix substrate type IV collagen and fibronectin, unlike parental SNB19 cells, which were smaller and spindle shaped. Biochemical studies showed that antisense uPAR-transfected cells, in addition to not spreading, exhibited increased expression of alpha 3 beta 1 integrin but not alpha 5 beta 1 integrin. However, we could not find a change in the expression of extracellular matrix components or altered growth rate in these cells. Furthermore, despite t...Continue Reading

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Citations

Dec 20, 2008·Journal of Neurochemistry·Manoranjan SantraMichael Chopp
Mar 25, 2014·Biochimica Et Biophysica Acta·Ilya UlasovCharles Cobbs
Dec 5, 1998·Microscopy Research and Technique·R H GoldbrunnerJ C Tonn
Jul 2, 2003·Nature Reviews. Cancer·Jasti S Rao
Jan 5, 2006·Brain Pathology·Sajani S LakkaJasti S Rao

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