Abstract
The interaction of urokinase-type plasminogen activator (uPA) with its cell-surface receptor (uPAR) is implicated in diverse biological processes such as cell migration, tissue remodeling, and tumor cell invasion. Recent studies indicated that uPAR can act as an extracellular matrix receptor during cell adhesion. Recently, we showed that transfection of the human glioma cell line SNB19 with antisense uPAR resulted in downregulation of uPAR at both the mRNA and protein levels. In this study, we used SNB19 to determine how the presence or absence of uPAR promotes cell spreading and associated changes in cell morphology. Microscopic analysis of cell spreading revealed that antisense uPAR-transfected cells were larger, remained round, and did not spread efficiently over extracellular matrix substrate type IV collagen and fibronectin, unlike parental SNB19 cells, which were smaller and spindle shaped. Biochemical studies showed that antisense uPAR-transfected cells, in addition to not spreading, exhibited increased expression of alpha 3 beta 1 integrin but not alpha 5 beta 1 integrin. However, we could not find a change in the expression of extracellular matrix components or altered growth rate in these cells. Furthermore, despite t...Continue Reading
References
Nov 1, 1992·The Journal of Cell Biology·K BurridgeL H Romer
Nov 1, 1992·Cancer Metastasis Reviews·H C Kwaan
Nov 6, 1992·Science·E J Luna, A L Hitt
Oct 1, 1991·The Journal of Cell Biology·P H QuaxJ H Verheijen
Jan 1, 1991·Advances in Cancer Research·J PöllänenA Vaheri
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·E B PasqualeS J Singer
Jan 1, 1985·Advances in Cancer Research·K DanøL Skriver
Apr 1, 1987·The Journal of Cell Biology·F BlasiK Danø
Apr 1, 1987·The Journal of Cell Biology·J PöllänenA Vaheri
Jan 1, 1988·The Journal of Cell Biology·J PöllänenA Vaheri
May 1, 1995·The Journal of Clinical Investigation·J NipP Brodt
Jun 1, 1995·Molecular Biology of the Cell·J A VarnerR L Juliano
Aug 1, 1995·Proceedings of the National Academy of Sciences of the United States of America·M M ZutterY L Tsung
Jan 1, 1994·Journal of Neuro-oncology·S MohanamJ S Rao
May 3, 1993·FEBS Letters·I DumlerW D Schleuning
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·C W CrowleyA D Levinson
Sep 1, 1993·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·H T MyöhänenA Vaheri
Jun 5, 1996·Cancer Letters·S K ChintalaJ S Rao
Sep 13, 1996·Science·Y WeiH A Chapman
Aug 1, 1996·Neurosurgery·A Giese, M Westphal
Nov 15, 1996·The Journal of Biological Chemistry·M YebraD A Cheresh
Mar 28, 1997·International Journal of Cancer. Journal International Du Cancer·A Stahl, B M Mueller
Nov 1, 1996·Frontiers in Bioscience : a Journal and Virtual Library·S K Chintala, J K Rao
Mar 20, 1997·Oncogene·S MohanamJ S Rao
Citations
May 30, 2012·International Journal of Oncology·Kiranmai AlapatiJasti S Rao
Dec 20, 2008·Journal of Neurochemistry·Manoranjan SantraMichael Chopp
Apr 13, 2012·Molecular Carcinogenesis·Rama Rao MallaJasti S Rao
Jun 15, 2000·Experimental Cell Research·K K HedbergO H Griffith
Mar 25, 2014·Biochimica Et Biophysica Acta·Ilya UlasovCharles Cobbs
Dec 5, 1998·Microscopy Research and Technique·R H GoldbrunnerJ C Tonn
Mar 29, 2005·Neurosurgery·Frederick F LangRaymond Sawaya
Jul 2, 2003·Nature Reviews. Cancer·Jasti S Rao
Jan 5, 2006·Brain Pathology·Sajani S LakkaJasti S Rao
Jul 29, 2000·Journal of Surgical Oncology·X ZhangY Chen
Nov 3, 2004·Journal of Cellular Physiology·Samah Abu-AliKanemitsu Shirasuna