Altered ionic effects of insulin in hypertension: role of basal ion levels in determining cellular responsiveness

The Journal of Clinical Endocrinology and Metabolism
M BarbagalloL M Resnick

Abstract

To investigate the ionic actions of insulin in hypertension, 19F- and 31P-nuclear magnetic resonance spectroscopy were used to measure cytosolic free calcium (Ca(i)) and intracellular free magnesium (Mg(i)) levels in red blood cells from normal (n = 9) and hypertensive (n = 9) subjects before and 30, 60, 120, and 180 min after in vitro incubation with insulin. In hypertensive patients, basal Ca(i) levels were significantly higher (30.0 +/- 2.2 vs. 19.8 +/- 2.5 nmol/L; P < 0.05), and basal Mg(i) levels were significantly lower (170 +/- 10.9 vs. 209 +/- 8 micromol/L; P < 0.05) than in normotensive subjects. In normal cells, insulin significantly elevated Ca(i) to 39.8 +/- 8.0, 50.1 +/- 8.2, 69.3 +/- 11.1, and 50.9 +/- 13.4 nmol/L at 30, 60, 120, and 180 min and Mg(i) to 238 +/- 10,264 +/- 14,226 +/- 11, and 216 +/- 10 micromol/L at 30, 60, 120, and 180 min. In hypertensive subjects, the insulin-dependent Ca(i) elevation was blunted, and Mg(i) accumulation was completely suppressed. Continuous relationships were observed between basal values of each ion and insulin responses; the greater the Ca(i), the less the Ca(i) rose (r = -0.574; P = 0.013), and the lower the Mg(i), the less Mg(i) rose (r = 0.524; P = 0.025). Furthermore, a b...Continue Reading

Citations

Aug 10, 2007·Current Hypertension Reports·Thomas D Giles, Gary E Sander
Mar 19, 2005·Clinical and Experimental Hypertension : CHE·William WeglickiLarry Resnick
Sep 19, 2006·Metabolism: Clinical and Experimental·Simona KratochvílováTerezie Pelikánová
Oct 3, 1999·Progress in Cardiovascular Diseases·L Resnick
Dec 17, 2004·Obesity Research·Jennifer A NasserPaul A Lachance
Jul 26, 2006·Blood Pressure·Pantelis A Sarafidis, Peter M Nilsson
Sep 13, 2000·Journal of the American Geriatrics Society·M BarbagalloL M Resnick
Jun 15, 2006·Nutrition in Clinical Practice : Official Publication of the American Society for Parenteral and Enteral Nutrition·A James HanjeCraig McClain
Jun 3, 2016·Scientific Reports·Wenxiang XiongZhao Wang
Apr 20, 2004·Journal of Cellular Physiology·Ana FerreiraJosé R Romero
Sep 22, 2001·Hypertension·M BarbagalloL M Resnick
Aug 2, 2007·American Journal of Therapeutics·Mario BarbagalloLawrence M Resnick
Jun 13, 2018·International Journal of Molecular Sciences·Krasimir Kostov, Lyudmila Halacheva
Jan 1, 2004·American Journal of Physiology. Endocrinology and Metabolism·Theresa E FaganAndrea Romani

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