Jan 22, 2014

Altered lipid composition in Streptococcus pneumoniae cpoA mutants

BMC Microbiology
Marina MeiersRegine Hakenbeck

Abstract

Penicillin-resistance in Streptococcus pneumoniae is mainly due to alterations in genes encoding the target enzymes for beta-lactams, the penicillin-binding proteins (PBPs). However, non-PBP genes are altered in beta-lactam-resistant laboratory mutants and confer decreased susceptibility to beta-lactam antibiotics. Two piperacillin resistant laboratory mutants of Streptococcus pneumoniae R6 contain mutations in the putative glycosyltransferase gene cpoA. The CpoA gene is part of an operon including another putative glycosyltransferase gene spr0982, both of which being homologous to glycolipid synthases present in other Gram-positive bacteria. We now show that the cpoA mutants as well as a cpoA deletion mutant are defective in the synthesis of galactosyl-glucosyl-diacylglycerol (GalGlcDAG) in vivo consistent with the in vitro function of CpoA as α-GalGlcDAG synthase as shown previously. In addition, the proportion of phosphatidylglycerol increased relative to cardiolipin in cpoA mutants. Moreover, cpoA mutants are more susceptible to acidic stress, have an increased requirement for Mg(2+) at low pH, reveal a higher resistance to lysis inducing conditions and are hypersensitive to bacitracin. The data show that deficiency of the ...Continue Reading

  • References41
  • Citations5

Citations

Mentioned in this Paper

Gram-Positive Bacteria
Pathologic Cytolysis
Vancomycin
Tetracycline Antibiotics
Gephyromantis luteus
Micrococcus luteus
Monobactams
Choline
Bacitracin
Phosphatidyl glycerol

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