Altered Lipid Synthesis by Lack of Yeast Pah1 Phosphatidate Phosphatase Reduces Chronological Life Span.

The Journal of Biological Chemistry
Yeonhee ParkG M Carman

Abstract

In Saccharomyces cerevisiae, Pah1 phosphatidate phosphatase, which catalyzes the dephosphorylation of phosphatidate to yield diacylglycerol, plays a crucial role in the synthesis of the storage lipid triacylglycerol. This evolutionarily conserved enzyme also plays a negative regulatory role in controlling de novo membrane phospholipid synthesis through its consumption of phosphatidate. We found that the pah1Δ mutant was defective in the utilization of non-fermentable carbon sources but not in oxidative phosphorylation; the mutant did not exhibit major changes in oxygen consumption rate, mitochondrial membrane potential, F1F0-ATP synthase activity, or gross mitochondrial morphology. The pah1Δ mutant contained an almost normal complement of major mitochondrial phospholipids with some alterations in molecular species. Although oxidative phosphorylation was not compromised in the pah1Δ mutant, the cellular levels of ATP in quiescent cells were reduced by 2-fold, inversely correlating with a 4-fold increase in membrane phospholipids. In addition, the quiescent pah1Δ mutant cells had 3-fold higher levels of mitochondrial superoxide and cellular lipid hydroperoxides, had reduced activities of superoxide dismutase 2 and catalase, and w...Continue Reading

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Citations

Aug 16, 2016·Journal of Lipid Research·J Pedro Fernández-Murray, Christopher R McMaster
Feb 10, 2018·Applied Microbiology and Biotechnology·Marianna DourouGeorge Aggelis
Jun 17, 2020·Journal of Lipid Research·Joanna M Kwiatek, George M Carman
Jul 22, 2018·Biotechnology for Biofuels·Xiao-Jing GuoYing-Jin Yuan
Jun 13, 2020·The Journal of Biological Chemistry·Mona MirheydariGeorge M Carman
Aug 8, 2020·Biotechnology for Biofuels·Jin-Lai ZhangYing-Jin Yuan
Sep 11, 2019·The Journal of Biological Chemistry·Prabuddha DeyGeorge M Carman
Sep 24, 2020·Journal of Lipid Research·Prabuddha DeyGeorge M Carman
Feb 4, 2021·Journal of Lipid Research·Prabuddha DeyGeorge M Carman
Aug 2, 2017·Biochemical and Biophysical Research Communications·Goldie Libby SherrChang-Hui Shen

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