PMID: 3827860Dec 15, 1986Paper

Altered lipid synthesis in type II pneumonocytes exposed to lung surfactant

The Biochemical Journal
N R ThakurJ E Bleasdale

Abstract

When type II pneumonocytes were exposed to purified lung surfactant that contained 1-palmitoyl-2-[3H]palmitoyl-glycero-3-phosphocholine, radiolabelled surfactant was apparently taken up by the cells since it could not be removed by either repeated washing or exchange with non-radiolabelled surfactant, but was released when the cells were lysed. After 4 h of exposure to [3H]surfactant, more than half of the 3H within cells remained in disaturated phosphatidylcholine. Incorporation of [3H]choline, [14C]palmitate and [14C]acetate into glycerophospholipids was decreased in type II cells exposed to surfactant and this inhibition, like surfactant uptake, was half-maximal when the extracellular concentration of surfactant was approx. 0.1 mumol of lipid P/ml. Inhibition of incorporation of radiolabelled precursors by surfactant occurred rapidly and reversibly and was not due solely to dilution of the specific radioactivity of intracellular precursors. Activity of dihydroxyacetone-phosphate acyltransferase, but not glycerol-3-phosphate acyltransferase, was decreased in type II cells exposed to surfactant and this was reflected by a decrease in the 14C/3H ratio of total lipids synthesized when cells incubated with [U-14C]glycerol and [2-...Continue Reading

Citations

Nov 1, 1992·Experimental Lung Research·P L DurhamJ M Snyder
Jul 24, 2013·Evidence-based Child Health : a Cochrane Review Journal·Esther CorenAnne J Martin
Oct 8, 1998·American Journal of Respiratory Cell and Molecular Biology·K L GossJ M Snyder
Feb 1, 1992·American Journal of Respiratory Cell and Molecular Biology·C L Wohlford-LenaneJ M Snyder
Aug 1, 1989·American Journal of Respiratory Cell and Molecular Biology·B E Miller, G E HooK
Sep 1, 1992·American Journal of Respiratory Cell and Molecular Biology·C L Wohlford-Lenane, J M Snyder
Mar 5, 1998·The American Journal of Physiology·A R Kumar, J M Snyder
Oct 10, 1998·Biochimica Et Biophysica Acta·O L MiakotinaJ M Snyder
Mar 15, 2001·Forensic Science International : Synergy·B L ZhuT Tsuji
Jan 1, 1988·General Pharmacology·T E Weaver
Jun 9, 1988·Biochimica Et Biophysica Acta·M Post, L M van Golde

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