PMID: 9190801Jun 1, 1997Paper

Altered localization of HrpZ in Pseudomonas syringae pv. syringae hrp mutants suggests that different components of the type III secretion pathway control protein translocation across the inner and outer membranes of gram-negative bacteria

Journal of Bacteriology
A O CharkowskiA Collmer

Abstract

Pseudomonas syringae pv. syringae 61 (Pss61) secretes the HrpZ harpin by a type III protein secretion pathway encoded by a cluster of hrp (hypersensitive response and pathogenicity) and hrc genes. The nine hrc genes represent a subset of hrp genes that are also conserved in the type III virulence protein secretion systems of animal pathogenic Yersinia, Shigella, and Salmonella spp. The hrpJ and hrpU operons contain seven hrc genes (counting hrcQ(A) and hrcQ(B) as one gene), all with additional homologs involved in flagellar biogenesis and secretion, and five of which encode predicted inner membrane proteins. The hrpC and hrpZ operons encode HrcC and HrcJ, respectively, which are associated with the outer membrane. Interposon mutants affected in all of the hrc genes in the hrpJ and hrpU operons and TnphoA-induced hrcC and hrcJ mutants were assayed for altered localization of HrpZ in mid-log-phase cultures by immunoblotting sodium dodecyl sulfate-polyacrylamide gels that were run with various cell fractions. The hrpJ and hrpU operon mutants revealed a novel phenotype of partially reduced accumulation of HrpZ in the total culture (despite wild-type levels of hrpZ operon transcription), all of which was cell bound and equivalent in...Continue Reading

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Citations

Mar 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·Ariel BlockerShin-Ichi Aizawa
Mar 12, 2004·Annual Review of Phytopathology·S W Hutcheson
May 13, 2010·Molecular Plant-microbe Interactions : MPMI·Hye-Sook OhAlan Collmer
Jan 28, 2015·Molecular Plant-microbe Interactions : MPMI·Andrew R RussellRoger W Innes
May 29, 2002·Proceedings of the National Academy of Sciences of the United States of America·Tanja Petnicki-OcwiejaJames R Alfano
Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·A CollmerJ R Alfano
Mar 1, 2004·Molecular Plant Pathology·Susan J Gropp, David S Guttman
Jun 7, 2014·Annual Review of Phytopathology·Jeff H ChangAllison L Creason
Aug 18, 1999·Proceedings of the National Academy of Sciences of the United States of America·S S HiranoC D Upper
Oct 26, 2010·Journal of Bacteriology·Dmitri V MavrodiLinda S Thomashow
Dec 25, 2003·Proceedings of the National Academy of Sciences of the United States of America·Vasiliki E FadouloglouMichael Kokkinidis
Jun 15, 2000·Journal of Bacteriology·E L HendricksonF M Ausubel
Sep 7, 2000·Microbiology and Molecular Biology Reviews : MMBR·S S Hirano, C D Upper
Aug 29, 1998·Journal of Bacteriology·G PrestonA Collmer
Jun 10, 1998·Microbiology and Molecular Biology Reviews : MMBR·C J Hueck
Dec 14, 2004·Microbiology and Molecular Biology Reviews : MMBR·Partho Ghosh

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